Structure of PDB 8br8 Chain LH Binding Site BS02

Receptor Information

>8br8 Chain LH (length=213) Species: 184922 (Giardia lamblia ATCC 50803)

ERATEKEKRAALVKDHAVKSAQVYARYYIDLVESEKKARENALKNNQLYV
PDEPKVYLVVRIRGIVGVAPKVRTILSLLRLRQINNAVFMRCNKATLNAL
RIVDHYVTYGEPTVETIRNLIYKRGFAKVNGQRIPIVDNNLIDEQLSKHN
ILCAEDLVHEIFTCGSSFKEANNFLWPFQLNSAALKDKRNNFAEGGDFGY
RGKYINEFVARMI

Ligand information

>8br8 Chain Lt (length=2593)

ggcgggccugaggcggcgggggcgacgggcggaacuuaagcauaucagua
cgccccggaggagaaaccaaccgggauuccccgcuagcggcgagcgacgc
gggaggagcccgccccgaaggcgcgcuguggggccgcgagggggcccgag
ggccccgcccgagagggugcaagccccguacggcgaguagcgcugcuuga
gcgugcagcgcgaagggaggcgcggcccuuccaaggcuaaauacgccccg
ggaccgauagcggaccaaguagcgcgagcgaacggugaaaaggacgcccu
gcggccgcucaaaagaccugaacccggccggccgccggcccgccggcccc
gucucgaaacacggaccgaggagccacgcgccgcggcgagcccgagggag
cccccgcggcggagcgagcgcgagacgccccgggcccgccaugccccugc
gggcgugcgcgggccgagccgcggcgcgugggcccgaaaggcggugaucu
augcccggcgagggcgaggccgggcgaaagccugguggaggcccgccgcg
gugcugacgcgcagaucgcucgucggagccgggcaugggggcgaaagacu
caucgaaccgccugguagcugguugccuccgaaaugucucccaggacagc
cgccgccccgcaguugcggcccguagagcgcuggccggcgggagcggggg
gccugccccucgcccgccccccaaacuccgaagggccgcgccgccccgcc
gcuggccugggcggggcgggcgaaugcgggcggcgcgugggccccuccug
guaagcaggacgggcgaggcgggacgauccggacgccgggccagggugcg
ccgccggggcccgcggaacggcgucggccggucccgacagccgaaucggc
cggcccggaaaauggagcgcgccggagccccggacccgcgcccggccgcc
gcgcgcggcggguaggaggccgcagaggccccgggggcgaaggcggcgcg
caggccccgccggaccggccucuggugcagaucucggcagcaguagccgc
uacuccgcgccccggaggacugagggggagacggguuccgcggcgccugc
aucuggccgcgggugacucgggccuaagcggcgggugaagaccgggaagg
ggcgcccgcccgucgaacggggagccggcggagacuccggcaggcgcggc
ccccgcggagacgcccgccccccggcgacgcgcacggggaccgcggcggg
cggcgccccggcccgcgaacgccccgcagcccccggacgccuugcgcgga
gaggggggcccgggggcggaccgcgcguccccggccgccccugaaaagcc
ggggggcgccggccgcgcgccguaccgaccgcagcaggacuccgggguca
gcagccucuagcgcgggagcgaacgcggcucagggaagucggcaagccgg
cuccguaaccucgggaaaaggaguggcucugacggcgccgggucagaacu
ggaacggacgcggggaucccgacuguuuacuagaaacacagcgucgcgag
ggccgcacccggcgcuggcgcgacgugauuucugcccagugccacgaccg
ucaccgugaagcgauccgccgaagcccugguaaacggcgggaguaacuau
gacucucuuaagguagccaaaugccucgucgggcaauuuccgacgugcau
gaauggaccaacgaggaucccacugucccgagccgcgccuccgcgagccu
ccagccucgggaacgggcgagggccggccagcggggcaagaagacccuuu
ugagcuugacuccagcccgggccuguggggcggggcggccggcgcagcgc
acaggggaggccgcgccccugagacacccugacggccgccgccgccccgc
ucacccggucgcgcggggacccgcccgggcggggaguucggcuggggcgg
cgcgccugcuacaccggaccgcaggcgucccacggcgggcucagcgagga
cggagaccucccgcggagcagaagggcacaagcccgcccgacccgcgccc
cccgugccggcgcgggccgcgaaagcggggccuaccgauccuucgccgcc
ccggccgcgggcgcggagguggcagaaaaguuaccacagggauaacuggc
uuguggccgccgagcgcccgcagcgacgcggcuuuuugauccuucgaugu
cggcucuuccuaccguccgcgcgcaccggcgcggaagcgucggauuguuc
acccguucaagggaucgugagcuggguuuagaccgucgugagacagguua
guuuuacccuacuggccccggggccagagcacggcgggccaguacgagag
gaacgcccgccgcgggccgccagccccgcgguugcccggccgggcagcgc
cgcgccgccgcgcccgggggcccugcgcugaccgccucuaagcgcgcacc
ccgccucgcgccccgcccggccgcgcgccccagccccgugccccgucgcc
gagcggcccccgcccggggagaccacccggcgcggcgcuccuguacggcg
cagagcccugcgaucgccugagggacgcgccugcagagcgcgg

.........................<<<<<<.....<<....>>......
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>>>>>..[[[...<<<<...........>>>><<<<....<<<<<.....
.>>>>>((......<<<.....))>>>...>>>>.....<<<<<<<....
....>>>>>>>...(((((..<<<<<<)))))..]]].............
.....................<<....>>................<<<..
..>>>............................<<<<<<..>>>>>>.<<
<<........>>>>.<<<<<....<<<<<<<<<<<<<..<<<...<<<..
..>>>..>>>...<<....>>............<<<<<<<<<<<<<....
>>>>>>>.>>>>>>...>>>>>>>>>>>>>..<<.<..<<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
<.<<.<<........>>>>>>>>....>>>>>>>>>...<<.......>>
......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><<
<<<<<<<<.<<<.<<<<<<<<<...<<<...<<<..<<<<<<..<<<<<<
......>>>>>>>>>>>>..>>>..>>.>...>>>>>>>.<<<<<<<<<<
.........>>>>>>>>>>...>>>>>>>>>>>>>.>>..<<<<.<<<<<
.....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((
...<<<<<<<........<<<.<<<<<<<<<..............>>>>>
>>>>>>>..........)))......>>>>>>>>>>>..>>>>>>><<<<
<....>>>>>....>>>>>>..<<<<<<<<...<<......<<<<<.<<.
...>>.>>>>>...>>>>>>>>>>........>>>>>.<<.....<<...
..<<<<<.....>>>>>..<<.....<<<<..<<...<<<<<<...<...
....>.>>>>>>....((<<<.<<...<<<...<<<....>>>......<
<....>>...>>.>....>>...<<<<<........>>>>>))<<<<<<<
<...<<<....>>>..<<<<<<<<<<...<<<..<<<<<<<<<<<<<<<<
<...{{{...>>>>>....<<<<<<...<<<<<<...<<<.....>>>..
...>>>>>>...>>>>>>..>>>>>.>>>>>>>..>>>.........>>>
>>>>>>>...>>>>>>>>.>>>..>>..<<......>>.>>>>.<<<<..
...>>>>..>>....<<<<....<<<<<<<<.<<.........<.....<
<<<<....<<..>>.....>>>>><<.<<<<<<<....>>..>>>>>.>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
.<<<<....>>>>......>>>>>>>...<<..<<<..<<<<<<.<...<
<....<<<.<....>.>>>....>>.>>>>>..>>>>><<<<<<......
.>>>>>>........<...........<<<<<<.....>>>>>>.....>
.....>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>....>>
.(..<<<<<......).>>>>>...((((((..{{{.........{{{..
{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<<<<<<<<.........
.<<<<<....................>>>>>.>>>>>>>>.>>>>>>>>>
...<<<<......>>>>.....>>>>>>>>..>>>>>...<<<.....>>
>..<<<<<<...........>>>>>>..<<...<<<<<<<...<.<<<<.
......>>>>.>.....<<...(((>>....>>>>>>>...<<<<<<<<.
........>>>>>>>>.<<<....>>>.>>)))......((((((.((((
(.>>>>>>))))))))))).........]]]]..}}.}}}.......<<.
<...<<<<<<...<<.......>>...>>>>>>....>.>.>.......<
<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<.<.....
>.>>>....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.
.......}}}.))))))..<<<<<..<<<..<<<<<<<<...........
....>>>>>>>><<<<<...<.<<...<<<<<<<<<<}}}.>>>>>..>>
>>>...>>.>.>>>>>.<<<...<<<<<<...........>>>>>>...>
>>..>>>..>>>>><...<<<<<<..........................
..>>>>>>..><<.<<<<.......>>>>..<<<<<<<<<<<....<<<<
....<<...>>...>>>>...>>>>.>>>>>.>>.....>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8br8 Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.
• Resolution: 3.35 Å
• Binding residue (original residue number in PDB): R85 G86 I87 V88 G89 P92 K93 R95 T96 I97 L100 R102 Q105 I106 R113 N115 K116 A117 N120 N152 Q154 R155 E166 Q167 K191 N195 Q201 L202 N203 S204 A206 K210 R211 R233
• Binding residue (residue number reindexed from 1): R63 G64 I65 V66 G67 P70 K71 R73 T74 I75 L78 R80 Q83 I84 R91 N93 K94 A95 N98 N130 Q132 R133 E144 Q145 K169 N173 Q179 L180 N181 S182 A184 K188 R189 R211

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit

External links

• PDB: RCSB:8br8, PDBe:8br8, PDBj:8br8
• PDBsum: 8br8
• PubMed: 36912103
• UniProt: A8BRY6