Structure of PDB 8rxx Chain LE Binding Site BS02

Receptor Information

>8rxx Chain LE (length=186) Species: 347515 (Leishmania major strain Friedlin)

VKVKSLCTLQIPEGVTVDVKGRKVTVTGKRGTLTKDLTHLQLDLRVDKKN
RTFTVIRWFGSKIPIACLNTTKAHVQNMITGVTKGYRFKVRCAYAHFPIN
VSVDGQNIEVRNFLGEKRVRRQLVPNTVKVSQTDPSKVKDEIVFDGNDLE
QVSREAAVLHQMCLVKKKDIRKFLDGIYVQTKTNIE

Ligand information

>8rxx Chain L2 (length=1149)

cccaacugcagaccguacucaucaccgcaucagguccccaagcauagaau
gcucugguacauagagaaagguacacucagggaagucggcaaaauagaug
cguaaguucgcaagaagcauuggcucugagggcaaaaaauugccaacuca
gaacugcuuacggcggggaauccaacuguauaauuaaaacauagguuugu
gaugcauccaagugguguauaucgcaaacugauuucugcccagugcucug
gucaacgugacgagauucaccgacgcgcggguaaacggcgggaguaacua
ugacucucuuaagguagccaaaugccucgucuuccaauuagagacgcgca
ugaauggauuaaugagauucccucugucccgaguuacuaucuagcgaaac
gacagucaagggaacggacuugaagggcuaagcggggaaagaagacccug
uugaguuugacuccagucuggcucugugcggcgacaucugaggcucggaa
cguugcuucacuuaucgaaugaagagaccaauggguuuagcguagucuuc
gggcuaugcaccgucuagguuugggaguccccuguggguacccaaccgug
cuguaaacugaaccaacgggaucaguuccggagucuuguuucgagacacu
gccagauggggaguuuggcuggggcggcauaucuguuacacgacaacgca
gguguccuaaggcgagcucagugggaacagaaaucucacguagaacacaa
gcguaaaagcuugcuugauuaacgauuuccaguacgaaucgagacugcga
aagcaaggccagcgauccuuugcgaagaauaggaaauaugaacaaaucau
accagaggugucagaauaauuaccacagggauaacuggcuuguggcggcc
aagcguucauagcgacgucgcuuuuugauccuucgaugucggcucuuccu
aaccuagcgccgcagaagacgcuaaggguuggauuguucacccacugaca
gggaacgugagcuggguuuagaccgucgugagacagguugguuuuacccu
acuuagcuggaaauugcgagaaaaagauuauccgugcaaagcggacgucg
ggucguugggccuggcgagcgguggguaguacaugguacgcucaucacc

........................<<......>>......<<<<.....>
>>>........<<<<....<<<<.<<<<.<<.........<.....<<<<
<...............>>>>><<.<<<<<<<<<<<....>>>>>..>>>>
>>.>>..........<<<<<<<<<..<<<.........>>>..<<<<<<<
....<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<..
<<....<<<<......>>>>....>>..>>>>..>>>>><<<<<<.....
..>>>>>>........<...........<<<<<.......>>>>>.....
>.....>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>.......
.....<<<<<........>>>>>..<<<<<<<..<<<.<<......<<<<
.<<<<<<..<<<<<.<<<<<<<...<<<.<<<<<<.<<<.<<.>>.>>>.
.>>>>>>.>>>...<<<<<.....<<<<....<<.....<<<<<<<<...
.>>>>>>>>.>>.....<<<<.<<...<<.<..>.>>...>>.>>>>...
................<<<<<....>>>>>..>>>>...>>>>>......
>>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>
>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<....
..>>....>>>>>>>...<<...<<<<<.......>>>>>..>>.<<<..
..>>>.>>........<<<<<<..............<<<<.......>>>
>.>>>>>>.........>>>>..>>>>>>.......<<.<...<<<<<<.
..<<.......>>...>>>>>>....>.>.>.......<<<<<.<<<<<<
..<<<<<<<.<......>.>>>>>..>>.<<<.........>>>.....>
>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>
.>>>>>>>.......................<<<<.....>>>>.<<<<<
<<<<....>>>>>>>>>.<.<<<<<<<.<<<<...>>>>>>>>>>>.>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8rxx Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.
• Resolution: 2.97 Å
• Binding residue (original residue number in PDB): K167 K169 D170 R172 F174
• Binding residue (residue number reindexed from 1): K166 K168 D169 R171 F173

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8rxx, PDBe:8rxx, PDBj:8rxx
• PDBsum: 8rxx
• PubMed: 38722744
• UniProt: Q4QC91