Structure of PDB 7zw0 Chain LE Binding Site BS02

Receptor Information

>7zw0 Chain LE (length=251) Species: 580240 (Saccharomyces cerevisiae W303)

GRVIRNQRKGAGSIFTSHTRLRQGAAKLRTLDYAERHGYIRGIVKQIVHD
SGRGAPLAKVVFRDPYKYRLREEIFIANEGVHTGQFIYAGKKASLNVGNV
LPLGSVPEGTIVSNVEEKPGDRGALARASGNYVIIIGHNPDENKTRVRLP
SGAKKVISSDARGVIGVIAGGGRVDKPLLKAGRAFHKYRLKRNSWPKTRG
VAMNPVDHPHGGGNHQHIGKASTISRGAVSGQKAGLIAARRTGLLRGSQK
T

Ligand information

>7zw0 Chain 2 (length=1765)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggccggucuuguaa
uuggaaugaguacaauguaaauaccuuaacgaggaacaauuggagggcaa
gucuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaa
guuguugcaguuaaaaagcucguaguugaacuuugggcccgguuggccgg
ucggauuuccaacggggccuuuccuucuggcuaaccuugagcuuggcgaa
ccaggacuuuuacuuugaaaaaauuagaguguucaaagcaggcguauugc
ucgaauauauuagcauggaauaauagaauaggacguuugguucuauuuug
uugguuucuaggaccaucguaaugauuaauagggacggucgggggcauca
guauucaauugucagaggugaaauucuuggauuuauugaagacuaacuac
ugcgaaagcauuugccaaggacguuuucauuaaucaagaacgaaaguuag
gggaucgaagaugaucagauaccgucguagucuuaaccauaaacuaugcc
gacuagggaucgggugguguuuuuuuaaugacccacucggcaccuuacga
gaaaucaaagucuuuggguucuggggggaguauggucgcaaggcugaaac
uuaaaggaauugacggaagggcaccaccaggaguggagccugcggcuuaa
uuugacucaacacggggaaacucaccagguccagacacaauaaggauuga
cagauugagagcucuuucuugauuuugugggugguggugcauggccguuc
uuaguugguggagugauuugucugcuuaauugcgauaacgaacgagaccu
uaaccuacuaaauaguggugcuagcauuugcugguuauccacuucuuaga
gggacuaucgguuucaagccgauggaaguuugaggcaauaacaggucugu
gaugcccuuagacguucugggccgcacgcgcgcuacacugacggagccag
cgagucuaaccuuggccgagaggucuugguaaucuugugaaacuccgucg
ugcuggggauagagcauuguaauuauugcucuucaacgaggaauuccuag
uaagcgcaagucaucagcuugcguugauuacgucccugcccuuuguacac
accgcccgucgcuaguaccgauugaauggcuuagugaggccucaggaucu
gcuuagagaagggggcaacuccaucucagagcggagaauuuggacaaacu
uggucauuuagaggaacuaaaagucguaacaagguuuccguaggugaacc
ugcggaaggaucauu

...<<<<<.{.[[[[>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..................<<....<<
.<<<.....>>>.>>.....>>.........<<<<<..<<....>>..>>
>>><...<<<<<<......(((((((.<<<....>>>....<........
>>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>
>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.......
...>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<
.....>>>..>>>..>....>.>.....<<<<<<<<<.<..>.>>>>>>.
>>>......<<..<...........>..>>.........<<<<<((....
<.<<<<.....<<..))>>.......>>>>>>>>>>..>>>>>>>>>>..
.......<<<[[.....<.<<...<<<.<<....<<<<<<.<<<<.....
.........>>>>.>>>>>>....<<<<<<....................
>>>>>>...<<<<<.<<.......<<...<.......>..<<<.....>>
>....>>......>>.>>..>>>.........<<<..<<<<<<<<.....
))))))).>>>>>>>>.>>>.....>>....<<<<<<.<<...<<<<..<
<..<<<<<<.<...<<<......>>>......>.>>>>>>..>>......
.<<....>>...>>>>...>>>>>.>>>...>>>...>>.>....<<<<<
<<...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<
.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>>...<<
..]]>>...>>.....>>>.>>>.<<<......<<<<....>>>>....>
>>..]]]].}<<<<<.<<<<<<<..<<.<<<<<<..<<<.<<<<<<....
..<<........>>..........<<<<<.<....<<<<<........<<
.<<<........>>>.>>......>>>>>...<<.<<<..<<.<<<<<<.
...<<<.<<<<<....>>>...<<<......>>>...>>.>>>....<<<
<<.<<..<<<<..<<<<<.<<<<<<....>>>>>>...>>>>>..>>>>.
>>....<<<<<<.....>>>>>>.......>>>>>....>>>.>>>....
.>>>>>>>.....>.>>>>>...>>.>>>>.>>>.....<<<<<<<....
..<<.....<<..<<<<....>>>>..>>....>>.......>>>>>>>.
..........<<<<<<..........>>>>>>..........>>>>>>..
..<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>.>>.
...<..<<.<..<<<<.<<....<<<<<<<<.<<<..<.<<..<...<<<
<<<<.<<<<.<<<<....>>>>.>>>>.>>>>>>>...>..>>.>..>>>
.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>
>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zw0 Sensing of individual stalled 80S ribosomes by Fap1 for nonfunctional rRNA turnover.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): R147 G248
• Binding residue (residue number reindexed from 1): R146 G247

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zw0, PDBe:7zw0, PDBj:7zw0
• PDBsum: 7zw0
• PubMed: 36113412
• UniProt: P0CX45|RL2A_YEAST Large ribosomal subunit protein uL2A (Gene Name=RPL2A)