Structure of PDB 8to1 Chain L Binding Site BS02

Receptor Information
>8to1 Chain L (length=551) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVM
EEAPAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTR
EGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLIT
GFVDIDPELAREKFAELRAQYVVTRDTIKHATAQEEILKLSEVFKQFRLV
PKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETS
DTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKDIN
RRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGL
MKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
LNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIG
DDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMR
FGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLD
D
Ligand information
Receptor-Ligand Complex Structure
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PDB8to1 Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy.
Resolution2.8 Å
Binding residue
(original residue number in PDB)		Q437 L573 E585 R588
Binding residue
(residue number reindexed from 1)		Q375 L511 E523 R526
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0005515 protein binding
GO:0016987 sigma factor activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006355 regulation of DNA-templated transcription
GO:0009408 response to heat
GO:0010468 regulation of gene expression
GO:0045892 negative regulation of DNA-templated transcription
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1903865 sigma factor antagonist complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8to1, PDBe:8to1, PDBj:8to1
PDBsum8to1
PubMed38951624
UniProtP00579|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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