Structure of PDB 7qgg Chain L Binding Site BS02

Receptor Information

>7qgg Chain L (length=170) Species: 10116 (Rattus norvegicus)

KENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTV
RSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGF
GIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRI
SKEEAMRWFQQKYDGIILPG

Ligand information

>7qgg Chain t (length=3679)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccgcgcgcggcgcgggaaaugug
gcguacggaagacccacuccccggcgccgcucguggggggcccaaguccu
ucugaucgaggcccagcccguggacggugugaggccgguagcggcccccg
gcgcgcgcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ccaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcgcgcguccggccgugccgguggccgg
cggaucuuucccgcucccggugucggcgggggaccgccccccggaucggc
gaccggccgccgccgggcgcacuuccaggcggugcgccgcgaccggcucc
gggacggcugggaaggcccggcggggaagguggcucggggccccgagugu
uacagccccccggcagcagcgcucgccgaaucccggggccgagggagccg
gauacccgucgccgcgcucuccccccggcgggggccgggccgccccuccc
acggcgcgaccgcucucccagggggcggggcggacuguccccagugcgcc
ccgggcgucgucgcgccgucgggcccgggcgcgcucucccuccccuucuc
ggggugggggggagcgagagcgcacggggucggcggcgaugucggcuacc
cacccgacccgucuugaaacacggaccaaggagucuaacgcgugcgcgag
ucaggggcucguccgaaagccgccguggcgcaaugaaggugaagggcccc
guucccgggggccgaggugggaucccgaggccucuccaguccgccgaggg
gcaccaccggcccgucucgcccgccgcgccggggagguggagcacgagcg
uacgcguuaggacccgaaagauggugaacuaugccugggcagggcgaagc
cagaggaaacucugguggagguccguagcgguccugacgugcaaaucggu
cguccgaccuggguauaggggcgaaagacuaaucgaaccaucuaguagcu
gguucccuccgaaguuucccucaggauagcuggcgcucucgcaacgcggu
cgacaacccgcaguuuuauccgguaaagcgaaugauuagaggucuugggg
ccgaaacgaucucaaccuauucucaaacuuuaaauggguaagaagcccgg
cucgcuggcguggagccgggcguggaaugcgagugccuagugggccacuu
uugguaagcagaacuggcgcugcgggaugaaccgaacgccggguuaaggc
gcccgaugccgacgcucaucagaccccagaaaagguguugguugauauag
acagcaggacgguggccauggaagucggaauccgcuaggaguguguaaca
acucaccugccgaaucaacuagcccugaaaauggauggcgcuggagcguc
gggcccauacccggccgucgccggcgucggaacgggacgggagcggccgc
ucgggccccgcggacgcuacgccgcgacgaguaggagggccgcugcggug
agccuugaagccuagggcgcgggcccggguggagccgccgcaggugcaga
ucuuggugguaguagcaaauauucaaacgagaacuuugaaggccgaagug
gagaaggguuccaugugaacagcaguugaacaugggucagucgguccuga
gagaugggcgagugccguuccgaagggacgggcgauggccuccguugccc
ucagccgaucgaaagggagucggguucagauccccgaauccggaguggcg
gagaugggcgccgcgaggcguccagugcgguaacgcgaccgaucccggag
aagccggcgggagccccggggagaguucucuuuucuuugugaagggcagg
gcgcccuggaauggguucgccccgagagaggggcccgugccuuggaaagc
gucgcaguuccggcggcguccggugagcucucgcuggcccuugaaaaucc
gggggagaggguguaaaucucgcgccgggccguacccauauccgcagcag
gucuccaaggugaacagccucuggcauguuggaacaauguagguaaggga
agucggcaagccggauccguaacuucgggauaaggauuggcucuaagggc
uggcagccgacuuagaacuggugcggaccaggggaauccgacuguuuaau
uaaaacaaagcaucgcgaaggcccgcggcggguguugacgcgaugugauu
ucugcccagugcucugaaugucaaagugaagaaauucaaugaagcgcggg
uaaacggcgggaguaacuaugacucucuuaagguagccaaaugccucguc
aucuaauuagugacgcgcaugaauggaugaacgagauucccacugucccu
accuacuauccagcgaaaccacagccaagggaacgggcuuggcggaauca
gcggggaaagaagacccuguugagcuugacucuagucuggcacggugaag
agacaugagagguguagaauaagugggaggcccgggccgccggugaaaua
ccacuacucucaucguuuuuucacugacccggugaggcgggggggcgagc
cccgaggggcucucgcuucuggcgccaagcgcccgucgggcgggcgcgac
ccgcuccggggacagugccagguggggaguuugacuggggcgguacaccu
gucaaacgguaacgcagguguccuaaggcgagcucagggaggacagaaac
cucccguggagcagaagggcaaaagcucgcuugaucuugauuuucaguac
gaauacagaccgugaaagcggggccucacgauccuucugaccuuuugggu
uuuaagcaggaggugucagaaaaguuaccacagggauaacuggcuugugg
cggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcuc
uuccuaucauugugaagcagaauucaccaagcguuggauuguucacccac
uaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuu
acccuacugaugauguguuguugccaugguaauccugcucaguacgagag
gaaccgcagguucagacauuugguguaugugcuuggcugaggagccaaug
gggcgaagcuaccaucugugggauuaugacugaacgccucuaagucagaa
ucccgcccaggcggaacgauacggcagcgccgaaggagccucgguuggcc
ccggauagccggcucccgggguccggugcggagagccauucgucccggga
aacggggugcggccggaaagggggccgcccucucgcccgucacgcuuaac
gcacguucguguggaaccuggcgcuaaaccauucguagacgaccugcuuc
ugggucgggguuucguacguagcagagcagcucccucgcugcgaucuauu
gaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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...(((((..<<<<<<)))))..]]]........................
..........<<....>>...............<<<<....>>>>.....
.............................<<<<<<<<..<<<.<.....<
..<<.<..<.<....<<.<..<..<.........<.<..<<<..<<..<<
<<<.<<<..<<<<<<<.....>..>>>>>>>>>>>>>>>>.>>>.>.>..
......>..>..>.>>....>.>..>.>>..>..<<<<<<<..<<<<<<<
<<<.<<<....<..<.......<<.<......<<<<<<<..>>>>>>>..
.......>>>......>......>.>>>...>>>>>>>>>>.........
.................<..<<<<<....>>>>>..<.<<.<.<<<.<<.
..<....<..<<<<...<<...>>..<<<<<<.<<<<....>>>>.>>>>
>>.>>>>...>....>....>>....>>>>>>..>.......<<<<<<<.
.....>>>>>>>.......>..........>>.>>>>>...>>>>...>>
>>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..<
<<...<<<<........>>>>.....>>>...<<....>>...<<.<<<<
<....>>>>>.>>..<<<<<..<<<...<....((((..[[..>...>>>
...>>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>
>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<
<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>>>.
>>>....>>>>>>>>>...<<.......>>......>>>>>>...>..>.
>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<..........<<
..>>.....<<<.<<<<<<<<<...<<<....<<<..<.<<<..<<<<<<
.........>>>>>>>>>.>..>>>..>.>>...>>>>>>>...<<<<<<
<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<<<<.<<
<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<
(((.......<<<<<.....<<..........<<<.<<<<<<<<<<....
...<<<<<.<<.....................>>........<.......
.>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>
..>>>...>>>>>>><<<<<<.<...........]]...)))).......
.....................>.>>>>>>....>>>>>>.<.<<<<<<<<
.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>....
.>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<.....<
<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...
<<....<<<....>>>..........<<.<<<<<<<<<...>>>>>>>>>
>>......>>....>>>..<<<<<........>>>>>))<<<<....<<<
<<..<<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<.
..<<<<<<<<<<<<<<<<<<<<<<<..{{{..>>>>>>....<<<<<.<<
<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>.....<<
<<<<<.......>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>>
>>>>..>..........>>.>>>.>>>>.>>>..>>.....<<......>
>>>>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<..
.............<<<<<....<<..>>.....>>>>><<.<<<<<<<<<
<<.>>>>>..>>>>>>.>>............<<<<<<<<<..<<<.....
....>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>...<
<..<<<..<<<<<<....<<<.....<<<<....>>>>.....>>>.>>>
>..>>>>><<<<<<.......>>>>>>........<...........<<<
<<.......>>>>>.....>.....>>....>>.>>>>>>>.....>>.>
>>>>>>..>>>>>>....>>....<<<<<........>>>>>....[[[[
[..{{{.{{......[[[[.{{[[[[..<<<<<.<<<<<<<<<.<<<<<<
<<<<<<<<<<<..........<<<.<..<<<<..>>>>............
>.>>>.>>>>>>>.>>>>>>>>>>...<<<<<.....<<<<....<<<..
.<.....>..>>..>.............<<<<<<<<...>>>>>>>>..>
>>>..>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<
<<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>>...<<<..<<<<.......
>>>>..>>>.<<<....>>>.>>))).......((((((..(((..>>>>
>>.)))))))))..........]]]]..}}]]]].......<<.<.<.<<
<<<<...<<.......>>...>>>>>>...>>.>.>.......<<<<<.<
<<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}
..}}}.]]]]].....<<<<<<.<..<<<..<.<<<<<............
.....>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>
>.>>...>>>>...>>>><<<<....<<<<<...........>>>>>...
.>>>>.>>>...>.>>>>>>......<<<<<...<...<.<.<<......
..........<.<<<..>>>.>..................<<<..<<<<.
........<<<<<..........>>>>>.......>>>>..>>>......
......>>..>.>...>..>>>>>.<<<<<.<.............>....
.<.<<<<<<<.<<.....<<<<.<..<<<<<......>>>>>..>>>>>.
...>>....>>>>.>>>.>...>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7qgg Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state.
• Resolution: 2.86 Å
• Binding residue (original residue number in PDB): K19 C21 N23 C25 S51 A53 R54 F60 A69 N97 N98 S100 D101 G106 F107 G127 D129 Y131 K145
• Binding residue (residue number reindexed from 1): K12 C14 N16 C18 S44 A46 R47 F53 A62 N90 N91 S93 D94 G99 F100 G120 D122 Y124 K138

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding
• GO:0031625 ubiquitin protein ligase binding
• GO:0042975 peroxisome proliferator activated receptor binding
• GO:1990948 ubiquitin ligase inhibitor activity

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0006605 protein targeting
• GO:0010628 positive regulation of gene expression
• GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process
• GO:0034504 protein localization to nucleus
• GO:0042273 ribosomal large subunit biogenesis
• GO:0050821 protein stabilization
• GO:0140236 translation at presynapse
• GO:0140242 translation at postsynapse
• GO:1901796 regulation of signal transduction by p53 class mediator
• GO:1901798 positive regulation of signal transduction by p53 class mediator
• GO:2000059 negative regulation of ubiquitin-dependent protein catabolic process
• GO:2000435 negative regulation of protein neddylation

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0032991 protein-containing complex
• GO:0045202 synapse
• GO:0098793 presynapse
• GO:0098794 postsynapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7qgg, PDBe:7qgg, PDBj:7qgg
• PDBsum: 7qgg
• PubMed: 36038000
• UniProt: P62914|RL11_RAT Large ribosomal subunit protein uL5 (Gene Name=Rpl11)