Structure of PDB 8txo Chain J Binding Site BS02

Receptor Information
>8txo Chain J (length=1162) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCARIFGPV
KDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIW
FLKSLPSRIGLLLDMPLRDIERVLYFEGAEAIQALLKSMDLEQECEQLRE
ELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLV
PLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAV
DALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRS
VITVGPYLRLHQCGLPKKMALELFKPFIYGVIREHPVLLNRAPTLHRLGI
QAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEARALM
MSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAE
RLYRSGLASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVP
KGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQIMYTGFAYAAR
SGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKVID
IWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSA
AQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLA
DTALKTANSGLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPL
RDRVLGRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSV
VSCDTDFGVCAHCYGRDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTF
HIGGASSIQVKNKGSIKLSNVKSVVNSSGKLVITSRNTELKLIDEFGRTK
ESYKVPYGAVLGETVANWDPHTMPGGLPRVADLFEARRPKEPAILAEISG
IVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERVERGDVIS
DGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQML
RKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITK
ASLATESFISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGT
GYAYHQDRMRRR
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8txo Overcoming resolution attenuation during tilted cryo-EM data collection.
Resolution3.1 Å
Binding residue
(original residue number in PDB)		R259 K334 R339 R346 R352 T790 A791 G794 L796 Q1326 E1327
Binding residue
(residue number reindexed from 1)		R206 K281 R286 R293 R299 T706 A707 G710 L711 Q1115 E1116
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0046677 response to antibiotic
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8txo, PDBe:8txo, PDBj:8txo
PDBsum8txo
PubMed38195598
UniProtP0A8T7|RPOC_ECOLI DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)

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