Structure of PDB 5hr4 Chain J Binding Site BS02

Receptor Information

>5hr4 Chain J (length=751) Species: 17 (Methylophilus methylotrophus)

KPQDPINIKAAERMGKLHDTLKLVGYEGHALELYLVRLLFCLFAEDTTIF
EKSLFQEYIETKTLEDGSDLAHHINTLFYVLNTPEQKRLKNLDEHLAAFP
YINGKLFEEPLPPAQFDKAMREALLDLCSLDWSRISPAIFGSLFQSIMDA
KKRRNLGAHYTSEANILKLIKPLFLDELWVEFEKVKNNKNKLLAFHKKLR
GLTFFDPACGCGNFLVITYRELRLLEIEVLRGLHRGGQQVLDIEHLIQIN
VDQFFGIEIEEFPAQIAQVALWLTDHQMNMKISDEFGNYFARIPLKSTPH
ILNANALQIDWNDVLEAKKCCFILGNPPFVGKSKQTPGQKADLLSVFGNL
KSASDLDLVAAWYPKAAHYIQTNANIRCAFVSTNSITQGEQVSLLWPLLL
SLGIKINFAHRTFSWTNEASGVAAVHCVIIGFGLKDSDEKIIYEYESING
EPLAIKAKNINPYLRDGVDVIACKRQQPISKLPSMRYGNKPTDDGNFLFT
DEEKNQFITNEPSSEKYFRRFVGGDEFINNTSRWCLWLDGADISEIRAMP
LVLARIKKVQEFRLKSSAKPTRQSASTPMKFFYISQPDTDYLLIPETSSE
NRQFIPIGFVDRNVISSNATYHIPSAEPLIFGLLSSTMHNCWMRNVGGRL
ESRYRYSASLVYNTFPWIQPNEKQSKAIEEAAFAILKARSNYPNESLAGL
YDPKTMPSELLKAHQKLDKAVDSVYGFKGPNTEIARIAFLFETYQKMTSL
L

Ligand information

>5hr4 Chain L (length=12)

ttatgtcggata

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5hr4 Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities.
• Resolution: 2.5964 Å
• Binding residue (original residue number in PDB): S488 K495 K506 K645 T647 D649 G679 D680 R688 S721 S722 A723 P725 T726 T752 S753 S754 R757 N773 A774 R808
• Binding residue (residue number reindexed from 1): S333 K340 K351 K490 T492 D494 G524 D525 R533 S566 S567 A568 P570 T571 T597 S598 S599 R602 N618 A619 R653

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:5hr4, PDBe:5hr4, PDBj:5hr4
• PDBsum: 5hr4
• PubMed: 27082731
• UniProt: B2MU09