Structure of PDB 5lzu Chain II Binding Site BS02

Receptor Information

>5lzu Chain II (length=206) Species: 9986 (Oryctolagus cuniculus)

GISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGG
NKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCI
VLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDE
RKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRK
IKARKG

Ligand information

>5lzu Chain 9 (length=1698)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
cccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggu
agucgccgugccuaccauggugaccacgggugacggggaaucaggguucg
auuccggagagggagccugagaaacggcuaccacauccaaggaaggcagc
aggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaaua
acaauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaa
auccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcg
guaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcu
cguaguuggaucuugggagcggccguccccugcucggcgccggcccgaag
cguuuacuuugaaaaaauuagaguguucaaagcaggccgccuggauaccg
cagcuaggaauaauggaauaggaccgcgguucuauuuuguugguuuucgg
aacugaggccaugauuaagagggacggccgggggcauucguauugcgccg
cuagaggugaaauucuuggaccggcgcaagacggaccagagcgaaagcau
uugccaagaauguuuucauuaaucaagaacgaaagucggagguucgaaga
cgaucagauaccgucguaguuccgaccauaaacgaugccgacuggcgaug
cggcggcguuauucccaugacccgccgggcagcuuccgggaaaccaaagu
cuuuggguuccggggggaguaugguugcaaagcugaaacuuaaaggaauu
gacggaagggcaccaccaggaguggagccugcggcuuaauuugacucaac
acgggaaaccucacccggcccggacacggacaggauugacagauugauag
cucuuucucgauuccgugggugguggugcauggccguucuuaguuggugg
agcgauuugucugguuaauuccgauaacgaacgagacucuggcaugcuaa
cuaguuacgcgaccccggucggcguaacuucuuagagggacaaguggcgu
ucagccacccgagauugagcaauaacaggucugugaugcccuuagauguc
cggggcugcacgcgcgcuacacugacuggcucagcgugugccuacccuac
gccggcaggcgcggguaacccguugaaccccauucgugauggggaucggg
gauugcaauuauuccccaugaacgaggaauucccaguaagugcgggucau
aagcuugcguugauuaagucccugcccuuuguacacaccgcccgucgcua
cuaccgauuggaugguuuagugaggcccucggaucggccccgccggggug
cccuggcggagcgcugagaagacggucgaacuugacuaucuagaggaagu
aaaagucguaacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<..........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<<.>>>>>>>>..>.>>>...>>..>.>>>.<<<....<<<....<<<<<
<<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>.
...>>>.>>.<<.<<<..........>>>.>>.<.<<....>>.>...>>
>>>>.........<<<....<<<<...>>>>..>>>..>...>>.>....
.<<<<<<<<<.<<<....>>>..>>>>>>.>>>......<<..<......
.....>..>>.........<<<<<((......<<<<.....<<..))>>.
......>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<
<...<<<.<<....<<<.<<<..>>>.>>><<<<<<<.<<..>>>>>>.>
>>.<<<<<<.<.......<<...<.......>.<<<<.>>>>...>>...
...>.>>>..>>>........<.<<.<<<<<<<...............>>
>>>>>.>>.>....>>....<<<<<<..<...<<<<..<<<.<<<<<<<<
...<<<......>>>......>>>>>>>>.>>>.......<<....>>..
.>>>>..>..>>>.>>>...>>>...>>.>....<<<<<<<...<...<<
<<.<.....>.>>>>...>>>>>>>>..........<<<.<<.<<<..<.
<<<<<<.<<<<......>>>>>>>>>>.>..>>>...<<..))>>...>>
.....>>>.>.>.<<<......<<<<....>>>>....>>>..)))).]<
<<<<.<<<<<<<..<<..<<<<<..<<<.<<<<<<......<<.......
.>>..........<<<<<.<....<<<<<<.......<<.<<<.......
.>>>.>>.....>>>>>>...<<.<<<..<<.<<<<<<....<<<.<<<<
<....>>>...<<<......>>>...>>.>>>....<<<...<...<<<<
..<<<<<<<<<<<<..>>>>.>>>>>>>>..>>>>..>.....<<<<<..
...>>>>>........>>>....>>>.>>>.....>>>>>>>.....>.>
>>>>...>>.>>>>.>>>.....<<.<<<........<.......<<<.<
<<<....>>>>.>>>....>.........>>>.>>......<...<.<<<
<<..........>>>>>>....>.....>>>>>.....<<<<<<<<....
...>>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<..<<
<<.<<....<<<<<<<<.<<<..<<<<.<<...<<<<<.<<<<<<<<<..
>>>>>>>>>...>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>
>...>.>>...>.....<<<<<<<<<<..>>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5lzu Decoding Mammalian Ribosome-mRNA States by Translational GTPase Complexes.
• Resolution: 3.75 Å
• Binding residue (original residue number in PDB): G2 R5 D6 N7 H9 K10 R11 R12 K13 T14 K19 Y21 H22 K23 K24 R25 K26 Y27 L29 R31 A34 R41 R42 I43 H44 T45 R47 V48 R49 G50 N52 K53 K54 Y55 R56 L58 N64 S66 T73 R74 K75 S86 N87 K98 N99 K144 I145 K147 K148 Y149 R178 Q181 R184 D186
• Binding residue (residue number reindexed from 1): G1 R4 D5 N6 H8 K9 R10 R11 K12 T13 K18 Y20 H21 K22 K23 R24 K25 Y26 L28 R30 A33 R40 R41 I42 H43 T44 R46 V47 R48 G49 N51 K52 K53 Y54 R55 L57 N63 S65 T72 R73 K74 S85 N86 K97 N98 K143 I144 K146 K147 Y148 R177 Q180 R183 D185

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5lzu, PDBe:5lzu, PDBj:5lzu
• PDBsum: 5lzu
• PubMed: 27863242
• UniProt: G1TJW1|RS8_RABIT Small ribosomal subunit protein eS8 (Gene Name=RPS8)