Structure of PDB 6gsl Chain I8 Binding Site BS02

Receptor Information

>6gsl Chain I8 (length=81) Species: 300852 (Thermus thermophilus HB8)

KKGLGSTRNGRDSQAKRLGVKRYEGQVVRAGNILVRQRGTRFKPGKNVGM
GRDFTLFALVDGVVEFQDRGRLGRYVHVRPL

Ligand information

>6gsl Chain 1H (length=2895)

aagaugguaagggcccacgguggaugccucggcacccgagccgaugaagg
acguggcuaccugcgauaagccagggggagccgguagcgggcguggaucc
cuggauguccgaaugggggaacccggccggcgggaacgccggucaccgcg
cuuuuugcgcggggggaaccuggggaacugaaacaucucaguacccagag
gagaggaaagagaaaucgacucccugaguagcggcgagcgaaaggggacc
agccuaaaccguccggcuuguccgggcggggucguggggcccucggacac
cgaauccccagccuagccgaagcuguugggaagcagcgccagagagggug
aaagccccguaggcgaaagguggggggauaggugaggguacccgaguacc
ccgugguucguggagccaugggggaaucugggcggaccaccgccuaaggc
uaaguacuccgggugaccgauagcgcaccaguaccgugagggaaagguga
aaagaaccccgggaggggagugaaauagagccugaaaccgugggcuuaca
agcagucacggccccgcaagggguuguggcgugccuauugaagcaugagc
cggcgacucacggucgugggcgagcuuaagccguugaggcggaggcguag
ggaaaccgaguccgaacagggcgcaaacgggggccgaaaaguccgcggcc
guggacccgaaaccgggcgagcuagcccuggccagggugaagcuggggug
agacccaguggaggcccgaaccggugggggaugcaaaccccucggaugag
cuggggcuaggagugaaaagcuaaccgagcccggagauagcugguucucc
ccgaaaugacuuuagggucagccucaggcgcugacuggggccuguagagc
acugauagggcuagggggcccaccagccuaccaaacccugucaaacuccg
aagggucccagguggagccugggagugagggcgcgagcgauaacguccgc
guccgagcgcgggaacaaccgagaccgccagcuaaggcccccaagucugg
gcuaagugguaaaggauguggcgccgcgaagacagccaggauguuggcuu
agaagcagccaucauuuaaagagugcguaauagcucacuggucgaguggc
gccgcgccgaaaaugaucggggcuuaagcccagcgccgaagcugcggguc
ugggggaugaccccaggcgguaggggagcguucccgaugccgaugaaggc
cgacccgcgagggcggcuggagguaagggaagugcgaaugccggcaugag
uaacgauaaagagggugagaaucccucucgccguaagcccaaggguuccu
acgcaauggucgucagcguaggguuaggcgggaccuaaggugaagccgaa
aggcguagccgaagggcagccgguuaauauuccggcccuucccgcaggug
cgauggggggacgcucuaggcuagggggaccggagccauggacgagcccg
gccagaagcgcagggugggagguaggcaaauccgccucccaacaagcucu
gcgugguggggaagcccguacgggugacaaccccccgaagccagggagcc
aagaaaagccucuaagcacaaccugcgggaacccguaccgcaaaccgaca
caggugggcgggugcaagagcacucaggcgcgcgggagaacccucgccaa
ggaacucugcaaguuggccccguaacuucgggagaaggggugcucccugg
ggugaugagccccggggagccgcagugaacaggcucuggcgacuguuuac
caaaaacacagcucucugcgaacucguaagaggagguauagggagcgacg
cuugcccggugccggaaggucaaggggaggggugcaagccccgaaccgaa
gccccggugaacggcggccguaacuauaacgguccuaagguagcgaaauu
ccuugucggguaaguuccgaccugcacgaaaagcguaacgaccggagcgc
ugucucggcgagggacccggugaaauugaacuggccgugaagaugcggcc
uacccguggcaggacgaaaagaccccguggagcuuuacugcagccuggug
uuggcucuuggucgcgccugcguaggauaggugggagccugugaaccccc
gccuccgggugggggggaggcgccggugaaauaccacccuggcgcggcug
ggggccuaacccucggauggggggacagcgcuuggcgggcaguuugacug
gggcggucgccuccuaaaagguaacggaggcgcccaaagguccccucagg
cgggacggaaauccgccggagagcgcaaggguagaagggggccugacugc
gaggccugcaagccgagcaggggcgaaagccgggccuagugaaccggugg
ucccguguggaagggccaucgaucaacggauaaaaguuaccccggggaua
acaggcugaucucccccgagcguccacagcggcggggagguuuggcaccu
cgaugucggcucgucgcauccuggggcugaagaaggucccaaggguuggg
cuguucgcccauuaaagcggcacgcgagcuggguucagaacgucgugaga
caguucggucucuauccgccacgggcgcaggaggcuugaggggggcucuu
ccuaguacgagaggaccggaagggacgcaccucugguuucccagcugucc
cuccaggggcauaagcuggguagccaugugcggaagggauaaccgcugaa
agcaucuaagcgggaagcccgccccaagaugaggccucccacggcgucaa
gccgguaaggacccgggaagaccacccgguggaugggccggggguguaag
cgccgcgaggcguugagccgaccggucccaaucguccgaggucuu

.<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<
<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>
>>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<
<.....>>>>>>.>>...<<<<<<.....<......>......>>>>>.>
.........<<....>>...<<<<<.....<<.....>>....>>>>>..
.<<<....<<<<<<<<.....>>>>>>>>......<<<<<<<<<....<<
<...<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<..
...))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<<
<<<<<<<.......>>>>>>>>>......<<<<<..((((>>>>>..>>>
......>>.>>>>>>.>>...............<<....>>....>>...
......<<<<....>>>>..................>>>>>>>>>>>...
....<<<<<<<<<<<....>>>>>>>>>>>...<............>.<<
<<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<
<....>>..<<<<.....>>>>.....<<.....>>....>>>>>>>>>>
>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<...
...>>>>>>.....>>>...<<<..<<<<........>>>>.>>>....>
>>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<
<<....<<<<<....>>>>><<<<<<<<<.....<<<<<<<<<...<<<.
..<<<<<<<<.<.<<<<<<......>>>>.>>.>.>>>>>>>>..>>.>.
..>>>>>>>>>.....>>>>>>>.>>..<<<<<<<.<<......>>.>>>
>>>>.....<<<......>>>...<<<<.<<<...<<<(((.....<<<<
<<...........<<..<<<<<<<<<........<<<<<.<....<<<<.
......>>>>.....>........<<......>>...>>>>>....>>>>
>>>>>.>>..........)))......>>>>>>.>>>...>>>>>>><<<
<<<<<.....>>>>>>>>....>>>>>>..<<<<<...<<<......<<<
<<.<<<....>>>>>>>>...>>>..>>>>>........>>>>>><....
..<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<
<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<...
.>>>...>>>...>>...<<<<<........>>>>>))<<<<<<<<<<<<
<...<<<<....<<<<<.<<<..<<<<<.<<<<.<<..[[[[...>>>>>
><<<...<<<<<<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>>
>>>>..>>><...<<<<....>>>>.....>>>>>>...>>>..>>>>>.
........>>>>...>>...>>>>>>>>>>>>>>>..>>.....<<....
..>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<<<<<<<.<
<.........<.....<<<<<.....<..>......>>>>><<<<<<<<<
<<<.....>>>>>>>>>>>>............<<<<<<<<<..<<<....
.....>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<
<<.<<<..<<<<<<<..<<<...<<...<<<<<....>>>>>...>>...
>>>>>>>>..>>>>><<<<<<.......>>>>>>........<.......
....<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..
>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>
..<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<.<<<.<<<<
<.<<<<<<.<<<<<<<<<...........<<...<..<<......<<<<<
<<<....>>>>>>>>...>>.............>..>>..>>>>>>>>>.
>>>>>>...<<<<<....>>>>>....>>>>>.>>>..>>>>>..<<<<.
....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<
<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<
..<<<......>>>..>>>>.<<<....>>>.>>))).......<<<<<<
<.<<..))))..>>>>>>>>>.............>>>>..>>>>>>....
...<<<<..<<<<<<<...<<.......>>...>>>>>>>...>>>.>..
.....<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<
<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>
>.>>>>........>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<
<<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<
<]]]]>>>>>>...>>>>>...>>>>.>>>>>...<<<....<<<<<...
........>>>>>....>>>.>>>>.......>>>>>>>..<<.......
..>>....<<<<<<<<<.......>>>>>....<<<<<<<<.<<<....<
<<<<....>>>>>...>>>..>>>>>.>>>...>>>>....>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6gsl Tautomeric G•U pairs within the molecular ribosomal grip and fidelity of decoding in bacteria.
• Resolution: 3.16 Å
• Binding residue (original residue number in PDB): T10 R11 N12 R14 D15 S16 Q17 A18 K19 R20 K24 R25 Y26 E27 Q29 A33 G34 N35 I36 R39 R41 G42 T43 R44 F45 G54 R55 D56 F57 F60 F69 R74 R77
• Binding residue (residue number reindexed from 1): T7 R8 N9 R11 D12 S13 Q14 A15 K16 R17 K21 R22 Y23 E24 Q26 A30 G31 N32 I33 R36 R38 G39 T40 R41 F42 G51 R52 D53 F54 F57 F66 R71 R74

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6gsl, PDBe:6gsl, PDBj:6gsl
• PDBsum: 6gsl
• PubMed: 29931292
• UniProt: P60493|RL27_THET8 Large ribosomal subunit protein bL27 (Gene Name=rpmA)