Structure of PDB 7oya Chain I2 Binding Site BS02

Receptor Information

>7oya Chain I2 (length=188) Species: 7955 (Danio rerio)

GISRDNWHKRRRTGGKRKPVHKKRKYELGRPAANTKIGPRRIHTIRVRGG
NKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCV
VLVDSTPYRQWYESHYALPLGRSKKVQKKFTLRRKTAKISPLLEEQFLQG
KLLACISSRPGQCGRADGYVLEGKELEFYLRKIKAKKG

Ligand information

>7oya Chain 51 (length=3321)

cacgaccucagcucagacgagaagacccgcugaauuuaagcauauuacua
agcggaggaaaagaaaccaaccgggauucccccaguagcggcgagcgaag
agggaaaaguccagcgccgaauccccgccccagggcgagggaccuguggc
guacggagggccgccucucucggcgcgggggccaaaguccuucugaugga
ggcuuagcccgcggacggugugaggccggugucggcccccgccccgccgg
ggugcgguuccucccggagucggguuguuugggaaugcagcccaaagcgg
gugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagcggac
aaguaccgugagggaaaguugaaaagaacuuugaagagagaguucaacag
ggcgugaaaccguuaagagguaaacggguggggaccgcaccguccgcccg
guggauucagcccggcggggcggggucggcccguccgguggcucccuucg
cucccgcgggggguugacgcccgggcgaaggcucggccgccgccgggugc
auuuccgccgcgguggagcgccgcgaccggcuccgguucggcuuggaagg
gucagggggcgaagguuuuacagcgcccucccgccccgacuucgccgcuu
gcucuccggggccgcgggcccucucugcccucccucugcggggacggggu
cccccgcccccggcguggcgcgacagggguggacuguccucaguccgcug
cgccgcgccgcccagggcgggggacccacguucgggcgcccgagguccgc
ggcgacgccggccucccacccgacccgucuugaaacacggaccaaggagu
ccaacgcgcgcgcgagucagaggguggcucgcgagccccugcggcgcaau
gaaggugagggugggauccccccgccccggcggggggcgcaccaccggcc
cgccucgcgacccuccggggggcagguggaguuggagcgcgcgcgauggc
acccgaaagauggugaacuaugccugggcagggcgaagccaggggaaacu
cugguggaggcccgccgcgguccugacgugcaaaucggucguccgaccug
ggcauaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccg
aaguuucccucaggauagcuggcgcucgccgaucaagcaguuuuauccgg
uaaagccaaugacuagaggcaccuauucucaaacuuuaaauggguaagag
gcccggcucgcuggcgcuggagccgggcguggaaugcgacgcgccuagug
ggccauuuuugguaagcagaacuggugcugcgggaugaaccgaacgccgg
guuaaggcgcccgaugccgacgcucaucagaccccauaaaagguguuggu
ugauauagacagcccgaaucaacuagcccugaaaauggauggcgcuggag
cgucgggcccauacccggccgucgacggcagcugcaagccucgacgagua
ggagggccgccgcgguggcgccgaagcccagggcgcgggcccggguggag
ccgccgcgggcgcagaucuuggugguaguagcaaauauucaaacgagagc
uuugaaggccgaaguggagaaggguuccaugugaacagcaguugaacaug
ggugagccgguccuaagggacgggccuacgccguucggaggggagggccc
cgcucgaccgaaagggagucggguccagauccccgagcccggagcggcgg
agacgggcgccgcgaggcgcccagugcggugacgcaaacgaacccggaga
ugccggcgggugccccgggaaagaguucucuuuucuuugugaagggcagg
gcgcccuggaacggguucgccccgagagaggggcccgcgcccuggaaagc
gccgcgcuucuggcggcguccggugagcucucgucggcccuugaaaaucc
gggggagaagguguaaaucucgcgccgggccguacccauauccgcagcag
gucuccaaggugaacagccucuggcguguuggaacaaggcagaguaaggg
aagucggcaagucagauccguaacuucgggauaaggauuggcucuaaggg
cugauagcagccagcuuagaacugucgcggaccaggggaauccgacuguu
uaauuaaaacaaagcaucgcgaaggcccucggcggguguugacgcgaugu
gauuucugcccagugcucugaaugucaaagugaagaaauucaacgaagcg
cggguaaacggcgggaguaacuaugacucucuuaagguagccaaaugccu
cgucaucuaauuagugacgcgcaugaauggaugaacgagauucccacugu
cccuaccugcuaucuagcgaaaccacagccaagggaacgggcuuggcaga
aucagcggggaaagaagacccuguugagcuugacucuagucuggcccugu
gaagagacuuuccucacuuacccggugaggcgggcuucuggcgucaagcg
ccccgcggggcgcgacccgccccggggacagcgucagguggggaguuuga
cuggggcgguacaccugucaaacgguaacgcagguguccuaaggcgagcu
caggggggacagaaaccucccguagagcagaagggcaaaagcucgcuuga
ucuugauuuucaguaugaguacggaccgcgaaagcggggccucacgaucc
uuccggcuuuugggguuuuaagcgggaggugucagaaaaguuaccacagg
gauaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuugau
ccuucgaugucggcucuuccuaucauugugaagcagaauucaccaagcgu
uggauuguucacccacuaacagggaacgugagcuggguuuagaccgucgu
gagacagguuaguuuuacccuacugaugugagccguuguugcaauaguaa
ucccgcucaguacgagaggaaccgcggguucagacauuugguucgugcgc
uuggcugaggagccacuggcgcgaagccaccaucugcgggauuaugacug
aacgccucuaagucagaaucccgccuaaaagcaacgauaagcgccgucgg
aucuccgauaggccccggguaaccgggaccccggcgcggagagccauucg
ugacaggagcggcuagaacgagcgccgcccccucuuccagugacgcaccg
cauguuuguggggaacccggugcuuaaaugacucguagacgaccugauuc
ugggucggggugucgugcguggcagagcagcgcugcgauccauugaaagu
cagcccucgauccaaguuuug

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<......<<.....>>....
.>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>
>>..<<<<<<<<<<...<<<.<..<.<<<<<<<.....<<<<......>>
>>.....((......<<<.....))>>>.....>>>>>>>...>..>.>>
>..>>>>>.>>>>>.......<<<<<<<........>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<<<<..<<<.......<<<<..
.<<<<....<<<<<<<<<.......<.<.<.<<<<.<..........<..
<.......>..>........>..>>>>..>..>.>....>>>>>>>>>..
...>>>>....>>>>.....<...<.<.<.<<<.<<...<....<<..<<
...<<<<.<<....<.>.....>>.>>>>....>>.........>>.>..
...>>..>>>.>.>........<.<<...<<<<.<..>.>>>>...<<<<
<..<<.<.<.<<<<<<...<<<<......<<<<<<<....>>>>>>>.>.
>>>.>>>>>>......>.>.>>>>>>>.......>>.>.......>....
>.......>>>...>>>>>>>>..<<<<........>>>><<<<<<....
<<<.<<<<<<<<<..<<<...<<<<.........>>>>....>>>...<<
....>>...<<<<<...<<<<<<<<....>>>>>>>>....>>>>><<<<
<<<<<.....<........>...>>>>>>.>>>...>>>>>>>>>.>>>.
.<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>
>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>
>>>>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<.
...<.<<<....>>>.><<.<<<<<<<.........<<<.<<<<<<<<<.
..<<<....<<<....<<<..>>>....>>>..>.>>...>>>>>>>...
<<<<<<<<<..........>>>>>>>>>....>>>>>>>.>>>>>..>>.
.<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<
<<...<<<(((.......<<<<<.....<<..........<<<.<<<<<<
<<<<.......<.>...>>>>>>>>>>>>>..........))).>>...>
>>>>..>>>...>>>>>>><<<<<<.<<<........>>>>>>>>>....
>>>>>>.<.<<<<<<<<<<......<<<<.<<<<....>>>>>>>>...>
>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<.......
>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>
>....((<<<<<<...<<....<<<....>>>............<...>.
.......>>....>>>..<<<<<........>>>>>))<<<<....<<<<
....<<<<<<<....>>>>>>>...<<<....>>>...<...<<<<<<..
.<<<<<<<<<.<<<<<<<.<<<<<<..[[[..>>>>>>....<<<<<.<<
<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>.....<<
<<<<<.......>>>>>>>>>>>.>.>>.>>>>>>>>>.........>>>
>>>...>..........>..>>>.>>>>.>>>..>>.....<<......>
>>>>>>.<<<<.....>>>>..>>>....<<<<....<<<<<.<<<.<<.
........<.....<<<<<.....<..>......>>>>><<.<<<<<<<<
<<<....>>>>>..>>>>>>.>>............<<<<<<<<<..<<<.
........>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>
...<<..<<<..<<<<<<....<<<.....<<<<....>>>>.....>>>
.>>>>..>>>>>.<<<<<.......>>>>>.........<..........
.<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>..
>>.>>>>>>>>.>>>>>>....>>....<<<<<........>>>>>....
<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<...<<
<<.<<<...>>>.>>>>...<<<<<.....<<<..............<<<
<<<<..>>>>>>>...>>>..>>>>>......>>>>>>>..>>>>>...<
<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<.
..<<<<<<.......>>>>>>.....<<.[.(((>>....>>>>>>>...
<<<..<<<<.......>>>>..>>>.<<<....>>>.>>)))].....<<
<<<<<..<<<..)))))).>>>>>>>>>>.........>>>>..>>>>>>
.......<<.<...<<<<<<...<<.......>>...>>>>>>....>.>
.>.......<<<<<.<<<<<<..<.<.<<<<<......>>>>>...>.>.
<<<.........>>>.....>>>>>..>...>>>.>>....<<<<<<<..
..>>>.>>>>....>>..>>>.>>>>>.......<<<<<<<<..<<<..<
.<<<<<.................>>>>>.><<<<....<<<<....<<<<
<<<<<.]]]..>>>>..>>>>>...>>>>...>>>><<<<<...<<<<<.
..........>>>>>...>>>>>.>>>...>>>>>>>>.<<<<<<...<.
..<<<<....<<..........<<<<<..>>>>>.........>>...<<
<.<..<<...<<<..........>>>.........>>...>.>>>.....
.........>>>>....>>>>>>>.<<<...<.............>....
<<<<<<<<<<..<.....<<<<....<<<<..>>>>...>>>>....>..
...>>>>.>>>>>>...>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7oya A molecular network of conserved factors keeps ribosomes dormant in the egg.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): N88 R92 K205
• Binding residue (residue number reindexed from 1): N87 R91 K186

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis
• GO:0043009 chordate embryonic development
• GO:0051726 regulation of cell cycle

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7oya, PDBe:7oya, PDBj:7oya
• PDBsum: 7oya
• PubMed: 36653451
• UniProt: P62247|RS8_DANRE Small ribosomal subunit protein eS8 (Gene Name=rps8)