Structure of PDB 5ui5 Chain I Binding Site BS02

Receptor Information

>5ui5 Chain I (length=316) Species: 224324 (Aquifex aeolicus VF5) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]

ELEELQQNIKLELEGKEQELALELLNYLNEKGFLSKSVEEISDVLRCSVE
ELEKVRQKVLRLEPLGVCSKDVWEFLELQIEEIYPEEEEILKKALRDLKR
GKKLKPEIKGKLSRLRLFPSAEKVYTFAKVDAIIEEENGEFFIYLYEDFI
DIDLNEEYWELYKNLQKELKEAFERYESIRKVLDIRRRNLRKVLEKIVER
QKDFLTGKGSLKPLTLREVSSEIGIHESTLSRIVNSKYVKTPVGTYSLRT
FFVRESAEGLTQGELMKLIKEIVERKPYSDQEIANILKEKGFKVARRTVA
KYREMLGIPSSRERRI

Ligand information

>5ui5 Chain B (length=30) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]

ctatttattgcaattttcgtgccaatttcg

Receptor-Ligand Complex Structure

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Structure summary

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PDB	5ui5 Crystal structure of Aquifex aeolicus sigma (N) bound to promoter DNA and the structure of sigma (N)-holoenzyme.
Resolution	3.4 Å
Binding residue (original residue number in PDB)	H304 S306 A377 R379 T380 Y384
Binding residue (residue number reindexed from 1)	H226 S228 A295 R297 T298 Y302
Binding affinity	PDBbind-CN: Kd=103nM

Enzymatic activity

Enzyme Commision number

Gene Ontology

	Molecular Function
GO:0001216	DNA-binding transcription activator activity
GO:0003677	DNA binding
GO:0016987	sigma factor activity

	Biological Process
GO:0006352	DNA-templated transcription initiation

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Molecular Function

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Biological Process

External links

PDB	RCSB:5ui5, PDBe:5ui5, PDBj:5ui5
PDBsum	5ui5
PubMed	28223493
UniProt	O66858

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