Structure of PDB 4xlr Chain I Binding Site BS02

Receptor Information
>4xlr Chain I (length=1117) Species: 271 (Thermus aquaticus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EIKRFGRIREVIPLPPLTEIQVESYKKALQADVPPEKRENVGIQAAFKET
FPIEEGDKGKGGLVLDFLEYRIGDPPFSQDECREKDLTYQAPLYARLQLI
HKDTGLIKEDEVFLGHLPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPD
PARPGRYIASIIPLPKRGPWIDLEVEASGVVTMKVNKRKFPLVLLLRVLG
YDQETLVRELSAYGDLVQGLLDEAVLAMRPEEAMVRLFTLLRPGDPPKKD
KALAYLFGLLADPKRYDLGEAGRYKAEEKLGVGLSGRTLVRFEDGEFKDE
VFLPTLRYLFALTAGVPGHEVDDIDHLGNRRIRTVGELMADQFRVGLARL
ARGVRERMVMGSPDTLTPAKLVNSRPLEAALREFFSRSQLSQFKDETNPL
SSLRHKRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGANIGL
ITSLAAYARVDALGFIRTPYRRVKNGVVTEEVVYMTASEEDRYTIAQANT
PLEGDRIATDRVVARRRGEPVIVAPEEVEFMDVSPKQVFSLNTNLIPFLE
HDDANRALMGSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDG
EVVKVDGTRIAVRYEDGRLVEHPLRRYARSNQGTAFDQRPRVRVGQRVKK
GDLLADGPASEEGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYT
SIHIERYEIEARDTKLGPERITRDIPHLSEAALRDLDEEGIVRIGAEVKP
GDILVGRTSFKGEQEPSPEERLLRSIFGEKARDVKDTSLRVPPGEGGIVV
GRLRLRRGDPGVELKPGVREVVRVFVAQKRKLQVGDKLANRHGNKGVVAK
ILPVEDMPHLPDGTPVDVILNPLGVPSRMNLGQILETHLGLAGYFLGQRY
ISPVFDGATEPEIKELLAEAFNLYFGKRQGEGFGVDKREKEVLARAEKLG
LVSPGKSPEEQLKELFDLGKVVLYDGRTGEPFEGPIVVGQMFIMKLYHMV
EDKMHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQE
MLTIKSDDIEGRNAAYQAIIKGEDVPEPSVPESFRVLVKELQALALDVQT
LDEKDNPVDIFEGLASK
Ligand information
>4xlr Chain S (length=48) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gcatccgtgagtcgagggtaataagcacaatttaacacttttgtcaag
Receptor-Ligand Complex Structure
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PDB4xlr CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.
Resolution4.3 Å
Binding residue
(original residue number in PDB)		R388 F394 K1004 G1023 K1024 Q1030 R1031 M1035
Binding residue
(residue number reindexed from 1)		R387 F393 K1003 G1022 K1023 Q1029 R1030 M1034
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4xlr, PDBe:4xlr, PDBj:4xlr
PDBsum4xlr
PubMed26349034
UniProtQ9KWU7|RPOB_THEAQ DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)

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