Structure of PDB 4k50 Chain I Binding Site BS02

Receptor Information

>4k50 Chain I (length=460) Species: 31708 (rhinovirus A16)

GQIQISKHVKDVGLPSIHTPTKTKLQPSVFYDIFPGSKEPAVLTEKDPRL
KVDFDSALFSKYKGNTECSLNEHIQVAVAHYSAQLATLDIDPQPIAMEDS
VFGMDGLEALDLNTSAGYPYVTLGIKKKDLINNKTKDISKLKLALDKYDV
DLPMITFLKDELRKKDKIAAGKTRVIEASSINDTILFRTVYGNLFSKFHL
NPGVVTGCAVGCDPETFWSKIPLMLDGDCIMAFDYTNYDGSIHPIWFKAL
GMVLDNLSFNPTLINRLCNSKHIFKSTYYEVEGGVPSGCSGTSIFNSMIN
NIIIRTLVLDAYKHIDLDKLKIIAYGDDVIFSYKYKLDMEAIAKEGQKYG
LTITPADKSSEFKELDYGNVTFLKRGFRQDDKYKFLIHPTFPVEEIYESI
RWTKKPSQMQEHVLSLCHLMWHNGPEIYKDFETKIRSVSAGRALYIPPYE
LLRHEWYEKF

Ligand information

>4k50 Chain J (length=33)

aggagaugaaagucuccaggucucucguccgga

.<<<<<<....>>>>>>................

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4k50 Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts.
• Resolution: 2.93 Å
• Binding residue (original residue number in PDB): H18 P20 K22 K24 E108 T114 S115 T122 K127 F157 K159 I176 A178 R188 H199 G211 C212 D213 G288 C289 Y325 E411 L414 H418
• Binding residue (residue number reindexed from 1): H18 P20 K22 K24 E108 T114 S115 T122 K127 F157 K159 I176 A178 R188 H199 G211 C212 D213 G288 C289 Y325 E411 L414 H418

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4k50, PDBe:4k50, PDBj:4k50
• PDBsum: 4k50
• PubMed: 23667424
• UniProt: Q82122|POLG_HRV16 Genome polyprotein