Structure of PDB 8u3b Chain F Binding Site BS02

Receptor Information
>8u3b Chain F (length=431) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPE
AITYLLEQYDRVEFVDPNAEELAREKFAELRAQYVVTRDTIKAKGRSHAT
AQEEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQ
CKMEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEA
NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYAT
WWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEE
LAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPL
DSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDV
TRERIRQIEAKALRKLRHPSRSEVLRSFLDD
Ligand information
>8u3b Chain 2 (length=69) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ccgctgccgcgaattccgtttcagggtacgcctgataatttgcattttaa
ataccatttattggttact
Receptor-Ligand Complex Structure
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PDB8u3b Structural basis for transcription activation by the nitrate-responsive regulator NarL.
Resolution3.23 Å
Binding residue
(original residue number in PDB)		Y394 N396 R397 G398 I443 K462 R465 I511 G512 D513 F522 L573 R588
Binding residue
(residue number reindexed from 1)		Y212 N214 R215 G216 I261 K280 R283 I329 G330 D331 F340 L391 R406
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003700 DNA-binding transcription factor activity
GO:0005515 protein binding
GO:0016987 sigma factor activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006355 regulation of DNA-templated transcription
GO:0009408 response to heat
GO:0010468 regulation of gene expression
GO:0045892 negative regulation of DNA-templated transcription
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1903865 sigma factor antagonist complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8u3b, PDBe:8u3b, PDBj:8u3b
PDBsum8u3b
PubMed38197271
UniProtP00579|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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