Structure of PDB 8jke Chain F Binding Site BS02

Receptor Information

>8jke Chain F (length=301) Species: 100226 (Streptomyces coelicolor A3(2))

ATADPVKDYLKQIGKVPLLNAEQEVELAKRIEAGLFAEDKLANSDKLAPK
LKRELEIIAEDGRRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNL
GLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVI
NKLARVQRQMLQDLGREPTPEELAKELDMTPEKVIEVQKYGREPISLHTP
LGEDGDSEFGDLIEDSEAVVPADAVSFTLLQEQLHSVLDTLSEREAGVVS
MRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQVLRDY
L

Ligand information

>8jke Chain P (length=61)

tgcgacggtctgacgctctacacagtgccagggggagataaacgaacgct
gaacgctccgg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8jke Structural and functional characterization of AfsR, a SARP family transcriptional activator of antibiotic biosynthesis in Streptomyces
• Resolution: 3.67 Å
• Binding residue (original residue number in PDB): R292 Y293 R296 W332 R335 Q336 R364 R367 I404 T408 P409 L410
• Binding residue (residue number reindexed from 1): R83 Y84 R87 W123 R126 Q127 R155 R158 I195 T199 P200 L201

Gene Ontology

Molecular Function

• GO:0001108 bacterial-type RNA polymerase holo enzyme binding
• GO:0003677 DNA binding
• GO:0003700 DNA-binding transcription factor activity
• GO:0005515 protein binding
• GO:0016987 sigma factor activity

Biological Process

• GO:0006352 DNA-templated transcription initiation
• GO:0006355 regulation of DNA-templated transcription
• GO:0010468 regulation of gene expression
• GO:2000142 regulation of DNA-templated transcription initiation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8jke, PDBe:8jke, PDBj:8jke
• PDBsum: 8jke
• PubMed: 38427710
• UniProt: P18183|SIGA_STRCO RNA polymerase principal sigma factor HrdB (Gene Name=hrdB)