Structure of PDB 7zux Chain ES Binding Site BS02

Receptor Information

>7zux Chain ES (length=159) Species: 4932 (Saccharomyces cerevisiae)

GKSHGYRSRTRYMFQRDFRKHGAVHLSTYLKVYKVGDIVDIKANGSIQKG
MPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSKC
RQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTL
APVPYETFI

Ligand information

>7zux Chain 5 (length=3163)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacgccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgauc
cugaugucuucggauggauuugaguaagagcauagcuguugggacccgaa
agauggugaacuaugccugaauagggugaagccagaggaaacucuggugg
aggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguauag
gggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuuc
ccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaaugau
uagagguuccggggucgaaaugaccuugaccuauucucaaacuuuaaaua
uguaagaaguccuuguuacuuaauugaacguggacauuugaaugaagagc
uuuuagugggccauuuuugguaagcagaacuggcgaugcgggaugaaccg
aacguagaguuaaggugccggaauacacgcucaucagacaccacaaaagg
uguuaguucaucuagacagccggacgguggccauggaagucggaauccgc
uaaggaguguguaacaacucaccggccgaaugaacuagcccugaaaaugg
auggcgcucaagcguguuaccuauacucuaccgucaggguugauaugaug
cccugacgaguaggcaggcguggaggucagugacgaagccuagaccguaa
ggucgggucgaacggccucuagugcagaucuuggugguaguagcaaauau
ucaaaugagaacuuugaagacugaaguggggaaagguuccacgucaacag
caguuggacguggguuagucgauccuaagagauggggaagcuccguuuca
aaggccugauuuuaugcaggccaccaucgaaagggaauccgguuaagauu
ccggaaccuggauauggauucuucacgguaacguaacugaauguggagac
gucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuauca
ccccggaauugguuuauccggagauggggucuuauggcuggaagaggcca
gcaccuuugcuggcuccggugcgcuugugacggcccgugaaaauccacag
gaaggaauaguuuucaugccaggucguacugauaaccgcagcaggucucc
aaggugaacagccucuaguugauagaauaauguagauaagggaagucggc
aaaauagauccguaacuucgggauaaggauuggcucuaagggucggguac
gaucaacuuagaacugguacggacaaggggaaucugacugucuaauuaaa
acauagcauugcgauggucagaaagugauguugacgcaaugugauuucug
cccagugcucugaaugucaaagugaagaaauucaaccaagcgcggguaaa
cggcgggaguaacuaugacucucuuaagguagccaaaugccucgucaucu
aauuagugacgcgcaugaauggauuaacgagauucccacugucccuaucu
acuaucuagcgaaaccacagccaagggaacgggcuuggcagaaucagcgg
ggaaagaagacccuguugagcuugacucuaguuugacauugugaagagac
auagagggcuaccuuuauaguuucuuuacuuauucaaugaagcggagcug
gaauucauuuuccacguucuagcauucaaggucccauucggggcugaucc
ggguugaagacauugucagguggggaguuuggcuggggcggcacaucugu
uaaacgauaacgcagauguccuaaggggggcucauggagaacagaaaucu
ccaguagaacaaaaggguaaaagcccccuugauuuugauuuucaguguga
auacaaaccaugaaaguguggccuaucgauccuuuagucccucggaauuu
gaggcuagaggugccagaaaaguuaccacagggauaacuggcuuguggca
gucaagcguucauagcgacauugcuuuuugauucuucgaugucggcucuu
ccuaucauaccgaagcagaauucgguaagcguuggauuguucacccacua
auagggaacgugagcuggguuuagaccgucgugagacagguuaguuuuac
ccuacugaugaauguuaccgcaauaguaauugaacuuaguacgagaggaa
caguucauucggauaauugguuuuugcggcugucugaucaggcauugccg
cgaagcuaccauccgcuggauuauggcugaacgccucuaagucagaaucc
augcuagaacgcggugauuucuuugcuccacacaauauagauggauacga
auaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggugc
acuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauuuu
gcguggggauaaaucauuuguauacgacuuagauguacaacgggguauug
uaagcaguagaguagccuuguuguuacgaucugcugagauuaagccuuug
uugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>...{.....<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<........<<.<<<<<<...>>>>>>.>>........
>....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]....}..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<....<<<<<<<.<<<<<
<<<<<<<<<<.....<<<<<<<<<......<<<<<......>>>>>....
...>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<
<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<<
....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<
<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<..
<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>..
...>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>..
.<<.......>>......>>>>>>...>..>.>>..<<<<<<....<.<<
<....>>>.><<<<<<<<<<....<<.<<<<<<<.....<<<....<<<.
...<<<<...<<<<<<.....>>>>>>.>>>>....>>>..>.>>.....
>>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>..>>>>
>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>
...<<<<<<<..<<<<(((.....<<<<<<.....<<..........<<<
.<<<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>.
........<<......>>...>>>>>...>>>>>>>>>>>>>........
..))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>
>>>>>>>>....>>>>>>..<<<<<<<<.<<......<<<<.<<<<....
>>>>>>>>...>>>>>>>>>>........>>>>>><<.....<<.....<
<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.
....>>.>>>>>>....((<<<<<<...<<....<<<....>>>......
..<<<<<<........>>>>>>....>>....>>...<<<<<........
>>>>>))<<<<.<<<<<<<.....<<<....>>>.......<<<<<<...
<<<<.<<<<<<<<<<<<<<<<<<..{{{..>>>>>><...<<<<<....<
<<<<<....<<<.....>>>....>>>>>>.....>>>>>....><<<<<
<<......>>>>>>>>>>>.>.>>>>>>>.>>>>.........>>>>>>.
.........>>>..>>>>>>>>>>>>..>>.....<<......>>>>>>>
.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.........
......<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<....>
>>>>..>>>>>>.>>............<<<<<<<<<..<<<.........
>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>...<<..<
<<..<<<<<<.....<<....<<<<<....>>>>.>...>>..>>>>..>
>>>><<<<<<.......>>>>>>........<...........<<<<<..
.....>>>>>.....>.....>>....>>.>>>>>>>.....>>.>>>>>
>>..>>>>>>....>>....<<<<<........>>>>>....[[[[[..{
{{.{{......[[[[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<
<<<<<<<....>>>>>>>.>>>>>>>>>>...<<<<<.....<<<<..<<
<<........>>>>...............<<<<<<....>>>>>>..>>>
>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<
...........>>>>>>..<<...<<<<<<<...<<<<<<.......>>>
>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<.......>>
>>.>>>>.<<<....>>>.>>))).......((((((..((((.>>>>>>
))))))))))..........]]]]..}}]]]].......<<.<.<.<<<<
<<...<<.......>>...>>>>>>...>>..>>.......<<<<<.<<<
<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>...
..>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}..
}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<..............
.>>>>>>.><<<<<...<<<<....<<<<<<<<<<}}}.>>>>>..>>>>
>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>
>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....<<......
....<<<<<<.....>>>>>>......>>.......<<<<<<...<<<<<
.<..<<<......>>>..>.>>>>>.>>>>>>.............>>.>>
>.>>>>>>..<<<<<.<.............>.....<<<<<<<<<.<<<.
...<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>>.>
>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zux Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 2.5 Å
• Binding residue (original residue number in PDB): G2 K3 S4 H5 G6 Y7 R8 R10 R12 Y13 M14 R17 F19 H22 G23 K35 V36 K43 S47 Q49 K50 G51 H54 F56 Y57 Q58 G59 K60 T61 T68 K69 S70 S71 K78 V80 N82 R83 L85 K87 R88 N90 R92 C101 R102 E104 F105 R108 K110 N112 R116 Q127 K129 R130
• Binding residue (residue number reindexed from 1): G1 K2 S3 H4 G5 Y6 R7 R9 R11 Y12 M13 R16 F18 H21 G22 K34 V35 K42 S46 Q48 K49 G50 H53 F55 Y56 Q57 G58 K59 T60 T67 K68 S69 S70 K77 V79 N81 R82 L84 K86 R87 N89 R91 C100 R101 E103 F104 R107 K109 N111 R115 Q126 K128 R129

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zux, PDBe:7zux, PDBj:7zux
• PDBsum: 7zux
• PubMed: 36801861
• UniProt: Q02753|RL21A_YEAST Large ribosomal subunit protein eL21A (Gene Name=RPL21A)