Structure of PDB 6z6b Chain EEE Binding Site BS02

Receptor Information

>6z6b Chain EEE (length=2031) Species: 11577 (La Crosse virus)

GMDYQEYQQFLARINTARDACVAKDIDVDLLMARHDYFGRELCKSLNIEY
RNDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSN
ESSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELY
PTIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCP
EFWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKK
DYSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLH
DQKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGK
MMDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQF
MINNDLIDKSEKLKLFKNFCGIGKHSKPKILDFDDANMYLASLTMMEQSK
KILSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFS
TLMKNILSVSQYNRHNTFRIAMCANNNVFAIVFPSAATVVYSIIVLHKEE
ENIFNPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLLF
KHDNPTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVKD
YIAEKFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDITQ
KGIKDNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLAK
TILEIECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHLR
NRIENRNNFRRSITTISTFTSSKSCLKIGDFRKEKELQSVKQKKILEVQS
RKMRLANPMFNYEMLRNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIE
QIMDMMIDHKKFYFTFFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKER
CKLNPDEMISEPGDGKLKVLEQKSEQEIRFLVETTRQKNREIDEAIEALA
SNLGKIEKLSLGKAKGLKMEINADMSKWSAQDVFYKYFWLIALDPILYPQ
EKERILYFMCNYMDKELILPDELLFNLLDQKVAYQNDIIATMTNQLNSNT
VLIKRNWLQGNFNYTSSYVHSCAMSVYKEILKEAITLLDGSILVNSLVHS
DDNQTSITIVQDKMENDKIIDFAMKEFERACLTFGCQANMKKTYVTNCIK
EFVSLFNLYGEPFSIYGRFLLTSVGDCAYIGPYEDLASRISSAQTAIKHG
CPPSLAWVSIAISHWMTSLTYNMLPGQSNDPIDYFPAENRKDIPIELNGV
LDAPLSMISTVGLESGNLYFLIKLLSKYTPVMQKRESVVNQIAEVKNWKV
EDLTDNEIFRLKILRYLVLDAGRSILTPRKFTTAGSLRKLYSFSKYQDRL
SSPGGMVELFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQ
NPAQLFIEQILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLE
LTNDDIQVIYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFR
SPALVLRAYSKNNPDTEMARDLVHLKEFVENTNLEEKMKVRIAMNEAEKG
QRDIVFELKEMTRFYQVCYEYVKSTEHKIKVFILPAKSYTTTDFCSLMQG
NLIKDKEWYTVHYLKQILSGGHKAINATSEQNIAFECFKLITHFADSFID
SLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLFRTGDLRQ
ADLDKYDAMKRILIRSLDYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVS
FEGPPIKTGLLDLTELMKSQDLLNLNYDNIRNSNLISFSKLICCEGSDNI
NDGLEFLSDDPMNFTEGEAIHSTPIFNIYYSKRGERHMTYRNAIKLLIER
ETKIFEEAFTFSENGFISPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHI
CLIKNGMDHMYHSFDVPKCFMGNPITRDINWVMFREFINSLPGTDIPPWN
VMTENFKKKCIALINSKFETQRDFSEFTKLM

Ligand information

>6z6b Chain CCC (length=16)

uuaguaguacacuacu

................

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6z6b Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
• Resolution: 3.961 Å
• Binding residue (original residue number in PDB): H312 N318 A320 K368 A371 R372 Q377 I378 N380 K381 K382 W395 Q397 Q398 L471 K472 Q506 N517 V521 Y524 R526 R531 M534 C535 A536 N537 N538 K859 K870 Q1313 S1314 I1511 L1512 F1513 H1515
• Binding residue (residue number reindexed from 1): H313 N319 A321 K369 A372 R373 Q378 I379 N381 K382 K383 W396 Q398 Q399 L459 K460 Q494 N505 V509 Y512 R514 R519 M522 C523 A524 N525 N526 K841 K852 Q1277 S1278 I1460 L1461 F1462 H1464

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:6z6b, PDBe:6z6b, PDBj:6z6b
• PDBsum: 6z6b
• PubMed: 32681014
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)