Structure of PDB 6gsl Chain E5 Binding Site BS02

Receptor Information

>6gsl Chain E5 (length=78) Species: 300852 (Thermus thermophilus HB8)

LGSTRNGRDSQAKRLGVKRYEGQVVRAGNILVRQRGTRFKPGKNVGMGRD
FTLFALVDGVVEFQDRGRLGRYVHVRPL

Ligand information

>6gsl Chain 14 (length=2895)

ggucaagaugguaagggcccacgguggaugccucggcacccgagccgaug
aaggacguggcuaccugcgauaagccagggggagccgguagcgggcgugg
aucccuggauguccgaaugggggaacccggccggcgggaacgccggucac
cgcgcuugcgcggggggaaccuggggaacugaaacaucucaguacccaga
ggagaggaaagagaaaucgacucccugaguagcggcgagcgaaaggggac
cagccuaaaccguccggcuuguccgggcggggucguggggcccucggaca
ccgaauccccagccuagccgaagcuguugggaagcagcgccagagagggu
gaaagccccguaggcgaaagguggggggauaggugaggguacccgaguac
cccgugguucguggagccaugggggaaucugggcggaccaccgccuaagg
cuaaguacuccgggugaccgauagcgcaccaguaccgugagggaaaggug
aaaagaaccccgggaggggagugaaauagagccugaaaccgugggcuuac
aagcagucacggccccgcaagggguuguggcgugccuauugaagcaugag
ccggcgacucacggucgugggcgagcuuaagccguugaggcggaggcgua
gggaaaccgaguccgaacagggcgcaaacggcgaaaaguccgcggccgug
gacccgaaaccgggcgagcuagcccuggccagggugaagcuggggugaga
cccaguggaggcccgaaccggugggggaugcaaaccccucggaugagcug
gggcuaggagugaaaagcuaaccgagcccggagauagcugguucuccccg
aaaugacuuuagggucagccucaggcgcugacuggggccuguagagcacu
gauagggcuagggggcccaccagccuaccaaacccugucaaacuccgaag
ggucccagguggagccugggagugagggcgcgagcgauaacguccgcguc
cgagcgcgggaacaaccgagaccgccagcuaaggcccccaagucugggcu
aagugguaaaggauguggcgccgcgaagacagccaggauguuggcuuaga
agcagccaucauuuaaagagugcguaauagcucacuggucgaguggcgcc
gcgccgaaaaugaucggggcuuaagcccagcgccgaagcugcgggucugg
gggaugaccccaggcgguaggggagcguucccgaugccgaugaaggccga
cccgcgagggcggcuggagguaagggaagugcgaaugccggcaugaguaa
cgauaaagagggugagaaucccucucgccguaagcccaaggguuccuacg
caauggucgucagcguaggguuaggcgggaccuaaggugaagccgaaagg
cguagccgaagggcagccgguuaauauuccggcccuucccgcaggugcga
uggggggacgcucuaggcuagggggaccggagccauggacgagcccggcc
agaagcgcagggugggagguaggcaaauccgccucccaacaagcucugcg
ugguggggaagcccguacgggugacaaccccccgaagccagggagccaag
aaaagccucuaagcacaaccugcgggaacccguaccgcaaaccgacacag
gugggcgggugcaagagcacucaggcgcgcgggagaacccucgccaagga
acucugcaaguuggccccguaacuucgggagaaggggugcucccuggggu
gaugagccccggggagccgcagugaacaggcucuggcgacuguuuaccaa
aaacacagcucucugcgaacucguaagaggagguauagggagcgacgcuu
gcccggugccggaaggucaaggggaggggugcaagccccgaaccgaagcc
ccggugaacggcggccguaacuauaacgguccuaagguagcgaaauuccu
ugucggguaaguuccgaccugcacgaaaagcguaacgaccggagcgcugu
cucggcgagggacccggugaaauugaacuggccgugaagaugcggccuac
ccguggcaggacgaaaagaccccguggagcuuuacugcagccugguguug
gcucuuggucgcgccugcuaggauaggugggagccugugaacccccgccu
ccgggugggggggaggcgccggugaaauaccacccuggcgcggcuggggg
ccuaacccucggauggggggacagcgcuuggcgggcaguuugacuggggc
ggucgccuccuaaaagguaacggaggcgcccaaagguccccucaggcggg
acggaaauccgccggagagcgcaaggguagaagggggccugacugcgagg
ccugcaagccgagcaggggcgaaagccgggccuagugaaccggugguccc
guguggaagggccaucgaucaacggauaaaaguuaccccggggauaacag
gcugaucucccccgagcguccacagcggcggggagguuuggcaccucgau
gucggcucgucgcauccuggggcugaagaaggucccaaggguugggcugu
ucgcccauuaaagcggcacgcgagcuggguucagaacgucgugagacagu
ucggucucuauccgccacgggcgcaggaggcuugaggggggcucuuccua
guacgagaggaccggaagggacgcaccucugguuucccagcugucccucc
aggggcauaagcuggguagccaugugcggaagggauaaccgcugaaagca
ucuaagcgggaagcccgccccaagaugaggccucccacggcgucaagccg
guaaggacccgggaagaccacccgguggaugggccggggguguaagcgcc
gcgaggcguugagccgaccggucccaaucguccgaggucuugacc

..............<<<<<<<<<<<.....[[..[[<<<<<<[[<<....
..<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<
<<<<....>>>>>.>>...<<<<<<.....<......>......>>>>>.
>.........<<....>>...<<<<<.....<<.....>>....>>>>>.
..(((....<<<<<<<<.....>>>>>>>>......<<<<<<<<<....<
<<...<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<.
....))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<
<<<<<<<<.......>>>>>>>>>......<<<<<..<<<<>>>>>..))
)......]].>>>>>>.]]...............<<....>>....]]..
.......<<<<....>>>>..................>>>>>>>>>>>..
.....<<<<<<<<<<<....>>>>>>>>>>>...<............>.<
<<<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...
<<....>>..<<<<.....>>>>..............>>>>>>>>>>>>>
..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<......
>>>>>>.....>>>...<<<..<<<<........>>>>.>>>....>>>>
>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<.
...<<<<<....>>>>><<<<<<<<<.....<<<<<<<<<...<<<...<
<<<<<<<.....<<<<......>>>>......>>>>>>>>..>>.>...>
>>>>>>>>.....>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>>
>.....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.
..........<<..<<<<<<<<<........<<<<<.<<...<<<<....
...>>>>...>>.........<<......>>...>>>>>....>>>>>>>
>>.>>..........)))......>>>>>>.>>>...>>>>>>><<<<<<
<.......>>>>>>>....>>>>>><.<<<<<...<<<......<<<<<.
<<<....>>>>>>>>...>>>..>>>>>..>.....>>>>>><......<
<.....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<
...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>
>...>>>...>>...<<<<<........>>>>>))<<<<<<<<<<<<<..
.<<<<....<<<<<.<<<..<<<<<.<<<<.<<..((((...>>>>>><<
<...<<<<<<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>>>>>
>..>>><...<<<<....>>>>.....>>>>>>...>>>..>>>>>....
.....>>>>...>>...>>>>>>>>>>>>>>>..>>.....<<......>
>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<<<<<<<.<<..
.......<.....<<<<<.....<..>......>>>>><<<<<<<<<<<<
.....>>>>>>>>>>>>............<<<<<<<<<..<<<.......
..>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.
<<<..<<<<<<<..<<<...<<...<<<<<....>>>>>...>>...>>>
>>>>>..>>>>><<<<<<.......>>>>>>........<..........
.<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..
>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<
<<<<<<<.(((.........<<<<.[[<<<<..<<<<<.<<<.<<<<<.<
<<<<<<<<<<<<<<<..........<<.....<<<......<<<<<<<<<
..>>>>>>>>>...>>>...............>>..>>>>>>>>>>>>>>
>>...<<<<<....>>>>>....>>>>>.>>>..>>>>>..<<<<.....
>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<
.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<
<......>>>..>>>>.<<<....>>>.>>))).......<<<<<<.(((
..>>>>..)))>>>>>>.............>>>>..]]>>>>.......<
<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>......
.<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<...
..>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>
>>........)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<..
.............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<)))
)>>>>>>...>>>>>...>>>>.>>>>>...<<<....<<<<........
.....>>>>....>>>.>>>>.......>>>>>>>..<<<.......>>>
....<<<<<<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<
....>>>>>...>>>..>>>>>.>>>...>>>>............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6gsl Tautomeric G•U pairs within the molecular ribosomal grip and fidelity of decoding in bacteria.
• Resolution: 3.16 Å
• Binding residue (original residue number in PDB): S9 N12 R14 D15 S16 K19 R20 K24 Y26 E27 Q29 A33 G34 N35 I36 R39 R41 G42 T43 R44 G54 R55 D56 F57 F60 F69 R74 R77
• Binding residue (residue number reindexed from 1): S3 N6 R8 D9 S10 K13 R14 K18 Y20 E21 Q23 A27 G28 N29 I30 R33 R35 G36 T37 R38 G48 R49 D50 F51 F54 F63 R68 R71

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6gsl, PDBe:6gsl, PDBj:6gsl
• PDBsum: 6gsl
• PubMed: 29931292
• UniProt: P60493|RL27_THET8 Large ribosomal subunit protein bL27 (Gene Name=rpmA)