Structure of PDB 4mz5 Chain E Binding Site BS02

Receptor Information

>4mz5 Chain E (length=427) Species: 10090 (Mus musculus)

QGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGL
IPKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISL
LASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLST
LACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLAD
SCWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNI
VTGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQI
QQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCG
IIEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGL
DKIEALQRHENESVYKASLNLIEKYFS

Ligand information

>4mz5 Chain C (length=12) Species: 9606 (Homo sapiens)

AISPSKRARPAE

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4mz5 Phosphorylation adjacent to the nuclear localization signal of human dUTPase abolishes nuclear import: structural and mechanistic insights.
• Resolution: 2.1 Å
• Binding residue (original residue number in PDB): S105 F138 W142 N146 S149 G150 T151 T155 Q181 W184 N188 G191 D192 N228 W231 R238 W273
• Binding residue (residue number reindexed from 1): S35 F68 W72 N76 S79 G80 T81 T85 Q111 W114 N118 G121 D122 N158 W161 R168 W203

Enzymatic activity

• Catalytic site (original residue number in PDB): T151 A163 N361 R366 Q367
• Catalytic site (residue number reindexed from 1): T81 A93 N291 R296 Q297

Gene Ontology

Molecular Function

• GO:0061608 nuclear import signal receptor activity

Biological Process

• GO:0006606 protein import into nucleus

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4mz5, PDBe:4mz5, PDBj:4mz5
• PDBsum: 4mz5
• PubMed: 24311590
• UniProt: P52293|IMA1_MOUSE Importin subunit alpha-1 (Gene Name=Kpna2)