Structure of PDB 4w2f Chain DS Binding Site BS02

Receptor Information

>4w2f Chain DS (length=110) Species: 300852 (Thermus thermophilus HB8)

RLTAYERRKFRVRNRIKRTGRLRLSVFRSLKHIYAQIIDDEKGVTLVSAS
SLALKLKGNKTEVARQVGRALAEKALALGIKQVAFDRGPYKYHGRVKALA
EGAREGGLEF

Ligand information

>4w2f Chain DB (length=120)

ucccccgugcccauagcggcguggaaccacccguucccauuccgaacacg
gaagugaaacgcgccagcgccgaugguacugggcgggcgaccgccuggga
gaguaggucggugcggggga

.<<<<<<<<<<....<<<<<<<<....<<<<<<...............>>
>..>>>...>>>>>>.>><<<.......<<<<<<<<....>>>>>>>>..
.....>>>.>>>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4w2f Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): R15 R25 F29 R30 S31 L32 K33 H34 Y36 Q38 G45 V46 T47 S50 A55 N61 K62 T63 Y92 H95 R97
• Binding residue (residue number reindexed from 1): R13 R23 F27 R28 S29 L30 K31 H32 Y34 Q36 G43 V44 T45 S48 A53 N59 K60 T61 Y90 H93 R95

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4w2f, PDBe:4w2f, PDBj:4w2f
• PDBsum: 4w2f
• PubMed: 25306919
• UniProt: Q5SHQ4|RL18_THET8 Large ribosomal subunit protein uL18 (Gene Name=rplR)