Structure of PDB 8e4p Chain D Binding Site BS02

Receptor Information
>8e4p Chain D (length=899) Species: 10090 (Mus musculus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
DLVNFIQANFKAFGDIQFGKYLRLSCDTDSETLYELLTQHWHLKTPNLVI
SVTGGAKNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKYIGEVV
RDNTISENIVAIGIAAWGMVSNRDTLFSAQYIMLYILDNNHTHLLLVDNG
CHGHPTVEAKLRNQLEKYISERTSQDSNYGGKIPIVCFAQGGGRETLKAI
NTSVKSKIPCVVVEGSGQIADVIASLVTSSMVKEKLVRFLPRTVSRLPEE
EIESWIKWLKEILESSHLLTVIKMEEAGDEIVSNAISYALYKAFSTNEQD
KDNWNGQLKLLLEWNQLDLASDEIFTNDRRWESADLQEVMFTALIKDRPK
FVRLFLENGLNLQKFLTNEVLTELFSTHFSTLVYRNLQIAKNSYNDALLT
FVWKLVANFRRTRHPLQALFIWAILQNKKELSKVIWEQTKGCTLAALGAS
KLLKTLAKVKNDINAAGESEELANEYETRAVELFTECYSNDEDLAEQLLV
YSCEAWGGSNCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKI
ILCLFIIPLVGCGLVSFRKKLLWYYVAFFTSPFVVFSWNVVFYIAFLLLF
AYVLLMDFHSVPHTPELILYALVFVLFCDEVRQWYMNGVNYFTDLWNVMD
TLGLFYFIAGIVFRLHSSNKSSLYSGRVIFCLDYIIFTLRLIHIFTVSRN
LGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEQRWRWIFRS
VIYEPYLAMFGEWITIPLVCIYMLSTNILLVNLLVAMFGYTVGIVQENND
QVWKFQRYFLVQEYCNRLNIPFPFVVFAYFYMVVKKCFKCCNEDNETLAW
EGVMKENYLVKINTKANDNSEEMRHRFRQLDSKLNDLKSLLKEIANNIK
Ligand information
Ligand IDY01
InChIInChI=1S/C31H50O4/c1-20(2)7-6-8-21(3)25-11-12-26-24-10-9-22-19-23(35-29(34)14-13-28(32)33)15-17-30(22,4)27(24)16-18-31(25,26)5/h9,20-21,23-27H,6-8,10-19H2,1-5H3,(H,32,33)/t21-,23+,24+,25-,26+,27+,30+,31-/m1/s1
InChIKeyWLNARFZDISHUGS-MIXBDBMTSA-N
SMILES
SoftwareSMILES
CACTVS 3.352CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@H](CC[C@]4(C)[C@H]3CC[C@]12C)OC(=O)CCC(O)=O
OpenEye OEToolkits 1.6.1CC(C)CCCC(C)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)OC(=O)CCC(=O)O)C)C
OpenEye OEToolkits 1.6.1CC(C)CCC[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)OC(=O)CCC(=O)O)C)C
CACTVS 3.352CC(C)CCC[CH](C)[CH]1CC[CH]2[CH]3CC=C4C[CH](CC[C]4(C)[CH]3CC[C]12C)OC(=O)CCC(O)=O
FormulaC31 H50 O4
NameCHOLESTEROL HEMISUCCINATE
ChEMBL
DrugBank
ZINCZINC000058638837
PDB chain8e4p Chain D Residue 1202 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8e4p Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP 2.
Resolution3.59 Å
Binding residue
(original residue number in PDB)		N692 W693 I696 F700
Binding residue
(residue number reindexed from 1)		N547 W548 I551 F555
Annotation score1
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0005216 monoatomic ion channel activity
GO:0005261 monoatomic cation channel activity
GO:0005262 calcium channel activity
GO:0005515 protein binding
GO:0042802 identical protein binding
Biological Process
GO:0006811 monoatomic ion transport
GO:0006816 calcium ion transport
GO:0006874 intracellular calcium ion homeostasis
GO:0009266 response to temperature stimulus
GO:0009409 response to cold
GO:0034220 monoatomic ion transmembrane transport
GO:0050955 thermoception
GO:0055085 transmembrane transport
GO:0070588 calcium ion transmembrane transport
GO:0120162 positive regulation of cold-induced thermogenesis
Cellular Component
GO:0005886 plasma membrane
GO:0009897 external side of plasma membrane
GO:0016020 membrane
GO:0044853 plasma membrane raft
GO:0045121 membrane raft

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8e4p, PDBe:8e4p, PDBj:8e4p
PDBsum8e4p
PubMed36227998
UniProtQ8R4D5|TRPM8_MOUSE Transient receptor potential cation channel subfamily M member 8 (Gene Name=Trpm8)

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