Structure of PDB 3px4 Chain D Binding Site BS02

Receptor Information

>3px4 Chain D (length=579) Species: 1462 (Geobacillus kaustophilus)

KMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFLR
PETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLLA
AYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHL
VRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDT
KRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPVL
KKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVRP
ATKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESDW
LIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDEV
TPNMRRQAKAVNYGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGVK
RYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPIQ
GSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCRL
VPEVMEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>3px4 Chain F (length=12)

ataggagtcagg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3px4 Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.
• Resolution: 1.582 Å
• Binding residue (original residue number in PDB): N527 N529 S530 K582 S585 T586 T611 Q612 T613 R615 S617 S618 T619 E620 N622 G711 Y714 I716 S717 Y719 G720 R729 R771 F781 N782 R789 N793 Q797
• Binding residue (residue number reindexed from 1): N230 N232 S233 K285 S288 T289 T314 Q315 T316 R318 S320 S321 T322 E323 N325 G414 Y417 I419 S420 Y422 G423 R432 R474 F484 N485 R492 N496 Q500

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:3px4, PDBe:3px4, PDBj:3px4
• PDBsum: 3px4
• PubMed: 22006298
• UniProt: Q5KWC1