Structure of PDB 2np6 Chain D Binding Site BS02

Receptor Information

>2np6 Chain D (length=393) Species: 271 (Thermus aquaticus)

VETPPEVVDFMVSLAEAPRGGRVLEPACAHGPFLRAFREAHGTAYRFVGV
EIDPKALDLPPWAEGILADFLLWEPGEAFDLILGNPPYGIVGEASKYPIH
VFKAVKDLYKKAFSTWKGKYNLYGAFLEKAVRLLKPGGVLVFVVPATWLV
LEDFALLREFLAREGKTSVYYLGEVFPQKKVSAVVIRFQKSGKGLSLWDT
QESESGFTPILWAEYPHWEGEIIRFETEETRKLEISGMPLGDLFHIRFAA
RSPEFKKHPAVRKEPGPGLVPVLTGRNLKPGWVDYEKNHSGLWMPKERAK
ELRDFYATPHLVVAHTKGTRVVAAWDERAYPWREEFHLLPKEGVRLDPSS
LVQWLNSEAMQKHVRTLYRDFVPHLTLRMLERLPVRREYGFHT

Ligand information

>2np6 Chain F (length=10)

gacatcgaac

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2np6 Differential binding of s-adenosylmethionine, s-adenosylhomocysteine and sinefungin to the adenine-specific DNA methyltransferase M.Taqi
• Resolution: 2.1 Å
• Binding residue (original residue number in PDB): G112 E113 K116 Y117 K126 K130 G138 K139 K200 R271 S272 G295 R296 T336 K337 R353 E354 P393
• Binding residue (residue number reindexed from 1): G92 E93 K96 Y97 K106 K110 G118 K119 K180 R251 S252 G275 R276 T316 K317 R333 E334 P373

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:2np6, PDBe:2np6, PDBj:2np6
• PDBsum: 2np6
• UniProt: P14385|MTTA_THEAQ Type II methyltransferase M.TaqI (Gene Name=taqIM)