Structure of PDB 2hvh Chain D Binding Site BS02

Receptor Information

>2hvh Chain D (length=580) Species: 1422 (Geobacillus stearothermophilus)

KKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PATKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNYGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>2hvh Chain F (length=11)

tgcgagtcagg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2hvh The structural basis for the mutagenicity of O6-methyl-guanine lesions.
• Resolution: 2.492 Å
• Binding residue (original residue number in PDB): N527 N529 S530 K582 T586 T611 Q612 T613 S617 S618 T619 E620 N622 G711 Y714 I716 S717 Y719 G720 R771 F786 R789 N793 Q797
• Binding residue (residue number reindexed from 1): N231 N233 S234 K286 T290 T315 Q316 T317 S321 S322 T323 E324 N326 G415 Y418 I420 S421 Y423 G424 R475 F490 R493 N497 Q501
• Binding affinity: PDBbind-CN: Kd=17.9uM

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:2hvh, PDBe:2hvh, PDBj:2hvh
• PDBsum: 2hvh
• PubMed: 17179038
• UniProt: Q45458