Structure of PDB 8wx0 Chain C Binding Site BS02

Receptor Information

>8wx0 Chain C (length=593) Species: 1773 (Mycobacterium tuberculosis)

IDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDNMLLSA
TTASKNPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCR
LIDRPLRPSFVDGLRNEIQIVVTILSLDPGDLYDVLAINAASASTQLGGL
PFSGPIGGVRVALIDGTWVGFPTVDQIERAVFDMVVAGRIVEGDVAIMMV
EAEATENVVELVEGGAQAPTESVVAAGLEAAKPFIAALCTAQQELADAAG
KSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQRIDEI
KTQVVQRLADTYEGREKEVGAALRALTKKLVRQRILTDHFRIDGRGITDI
RALSAEVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLGPETSK
RYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYA
IRQVSEALGSNGSTSMGSVCASTLALLNAGVPLKAPVAGIAMGLVSDDIQ
VEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLD
GIPSQVLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVT

Ligand information

>8wx0 Chain G (length=3)

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 8wx0 Cryo-EM structures of Mycobacterium tuberculosis polynucleotide phosphorylase suggest a potential mechanism for its RNA substrate degradation.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): F68 T72 R105 R112 R430
• Binding residue (residue number reindexed from 1): F63 T67 R100 R107 R425

Gene Ontology

Molecular Function

• GO:0000175 3'-5'-RNA exonuclease activity
• GO:0000287 magnesium ion binding
• GO:0003723 RNA binding
• GO:0004654 polyribonucleotide nucleotidyltransferase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006401 RNA catabolic process
• GO:0006402 mRNA catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:8wx0, PDBe:8wx0, PDBj:8wx0
• PDBsum: 8wx0
• PubMed: 38395123
• UniProt: P9WI57|PNP_MYCTU Polyribonucleotide nucleotidyltransferase (Gene Name=pnp)