Structure of PDB 8fs2 Chain C Binding Site BS02

Receptor Information

>8fs2 Chain C (length=537) Species: 1496 (Clostridioides difficile)

YTPKIIVDYIVKKTLKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLF
EENIYELKKKYDENYWTVDNIHRHILNYCIYGADIDEKAISILKDSLTNK
IKINLFCCDSLKKKWRYKFDYIVGNPPYIGHKKLEKKYKKFLLEKYSEVY
KDKADLYFCFYKKIIDILKQGGIGSVITPRYFLESLSGKDLREYIKSNVN
VQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYIDVFKIKNEDICINKF
ETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKCKYSLED
IAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKS
EYRLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWG
REKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSY
EYLVGILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIE
NLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLGI

Ligand information

>8fs2 Chain I (length=14)

atgggactttttga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fs2 Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase.
• Resolution: 2.59 Å
• Binding residue (original residue number in PDB): H171 K172 K179 D192 K193 I349 G351 C352 D353 K378 K380 R425 Q438 W439 Y455
• Binding residue (residue number reindexed from 1): H131 K132 K139 D152 K153 I309 G311 C312 D313 K338 K340 R385 Q398 W399 Y415

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0032259 methylation

External links

• PDB: RCSB:8fs2, PDBe:8fs2, PDBj:8fs2
• PDBsum: 8fs2
• PubMed: 37082867
• UniProt: Q183J3