Structure of PDB 7rfm Chain C Binding Site BS02

Receptor Information

>7rfm Chain C (length=537) Species: 272563 (Clostridioides difficile 630)

YYTPKIIVDYIVKKTLKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDL
FEENIYELKKKYDENYWTVDNIHRHILNYCIYGADIDEKAISILKDSLTN
IKINLFCCDSLKKKWRYKFDYIVGNPPYIGHKKLEKKYKKFLLEKYSEVY
KDKADLYFCFYKKIIDILKQGGIGSVITPRYFLESLSGKDLREYIKSNVN
VQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYIDVFKIKNEDICINKF
ETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKCKYSLED
IAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKS
EYRLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWG
REKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSY
EYLVGILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIE
NLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLGI

Ligand information

>7rfm Chain I (length=14)

atgggactttttga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7rfm Repurposing epigenetic inhibitors to target the Clostridioides difficile- specific DNA adenine methyltransferase and sporulation regulator CamA.
• Resolution: 2.68 Å
• Binding residue (original residue number in PDB): K172 K179 D192 K193 I349 G351 D353 K378 S379 K380 R425 Q438 W439 Y455 A473
• Binding residue (residue number reindexed from 1): K132 K139 D152 K153 I309 G311 D313 K338 S339 K340 R385 Q398 W399 Y415 A433

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0032259 methylation

External links

• PDB: RCSB:7rfm, PDBe:7rfm, PDBj:7rfm
• PDBsum: 7rfm
• PubMed: 34523387
• UniProt: Q183J3