Structure of PDB 5btr Chain C Binding Site BS02

Receptor Information

>5btr Chain C (length=322) Species: 9606 (Homo sapiens)

DNLLFGDEIITNDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPP
PELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQESKKIIVLTGY
ARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFI
ALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDC
EAVRGDIFNQVVPRCAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVI
GSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINEL
CHRLGGEYAKLSFLPPNRYIFH

Ligand information

>5btr Chain F (length=4) Species: 32630 (synthetic construct)

RHKA

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5btr Structural basis for allosteric, substrate-dependent stimulation of SIRT1 activity by resveratrol
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): T209 N226 F297 H363 V412 F413 F414 E416 N417 R446 P447
• Binding residue (residue number reindexed from 1): T47 N64 F117 H183 V223 F224 F225 E227 N228 R257 P258

Enzymatic activity

• Catalytic site (original residue number in PDB): N346 D348 H363
• Catalytic site (residue number reindexed from 1): N166 D168 H183
• Enzyme Commision number: 2.3.1.-
  2.3.1.286: protein acetyllysine N-acetyltransferase.

Gene Ontology

Molecular Function

• GO:0070403 NAD+ binding

External links

• PDB: RCSB:5btr, PDBe:5btr, PDBj:5btr
• PDBsum: 5btr
• PubMed: 26109052
• UniProt: Q96EB6|SIR1_HUMAN NAD-dependent protein deacetylase sirtuin-1 (Gene Name=SIRT1)