Structure of PDB 3n25 Chain C Binding Site BS02

Receptor Information
>3n25 Chain C (length=512) Species: 9986 (Oryctolagus cuniculus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
IQTQQLHAAMADTFLEHKCRLDIDSAPITARNTGIICTIGPASRSVETLK
EMIKSGMNVARMNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVAL
DTKGPEIRTGLIAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKV
VDVGSKVYVDDGLISLQVKQKDFLVTEVENGGFLGSKKGVNLPGAAVDLP
AVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKILGEKGKNIKIIS
KIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMIIGRCNR
AGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGD
YPLEAVRMQHLIAREAEAAMFHRKLFEELARASSQSTDLMEAMAMGSVEA
SYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNHQTARQAHLYRGI
FPVVCKDPVQEAWAEDVDLRVNLAMNVGKARGFFKKGDVVIVLTGWRPGS
GFTNTMRVVPVP
Ligand information
Ligand IDMN
InChIInChI=1S/Mn/q+2
InChIKeyWAEMQWOKJMHJLA-UHFFFAOYSA-N
SMILES
SoftwareSMILES
ACDLabs 10.04
OpenEye OEToolkits 1.5.0		[Mn+2]
CACTVS 3.341[Mn++]
FormulaMn
NameMANGANESE (II) ION
ChEMBL
DrugBankDB06757
ZINC
PDB chain3n25 Chain C Residue 640 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB3n25 The pyruvate kinase model system, a cautionary tale for the use of osmolyte perturbations to support conformational equilibria in allostery.
Resolution2.41 Å
Binding residue
(original residue number in PDB)		E271 D295
Binding residue
(residue number reindexed from 1)		E253 D277
Annotation score1
Enzymatic activity
Catalytic site (original residue number in PDB) R72 R119 K269 T327
Catalytic site (residue number reindexed from 1) R61 R108 K251 T309
Enzyme Commision number 2.7.10.2: non-specific protein-tyrosine kinase.
2.7.11.1: non-specific serine/threonine protein kinase.
2.7.1.40: pyruvate kinase.
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003729 mRNA binding
GO:0003824 catalytic activity
GO:0004713 protein tyrosine kinase activity
GO:0004743 pyruvate kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0030955 potassium ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006096 glycolytic process
GO:0006417 regulation of translation
GO:0016310 phosphorylation
GO:0032869 cellular response to insulin stimulus
GO:1903672 positive regulation of sprouting angiogenesis
GO:2000767 positive regulation of cytoplasmic translation
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005791 rough endoplasmic reticulum

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3n25, PDBe:3n25, PDBj:3n25
PDBsum3n25
PubMed20629175
UniProtP11974|KPYM_RABIT Pyruvate kinase PKM (Gene Name=PKM)

[Back to BioLiP]