Structure of PDB 1s0v Chain C Binding Site BS02

Receptor Information

>1s0v Chain C (length=857) Species: 10760 (Escherichia phage T7)

NTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARF
RKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPT
AFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARF
GRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAW
SSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYA
EAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHS
KKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVED
IPAIEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFP
YNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANC
AGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE
YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETV
QDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALA
GQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMF
TQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGE
ILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRDSEIDAHKQES
GIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFK
AVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDIL
ESDFAFA

Ligand information

>1s0v Chain L (length=8)

gcggcgau

........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1s0v Structural basis for substrate selection by t7 RNA polymerase.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): K172 K389 A390 S393 R394 R425 G436 Y639 D812
• Binding residue (residue number reindexed from 1): K171 K372 A373 S376 R377 R408 G419 Y622 D786

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription

External links

• PDB: RCSB:1s0v, PDBe:1s0v, PDBj:1s0v
• PDBsum: 1s0v
• PubMed: 15016373
• UniProt: P00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)