Structure of PDB 8ove Chain Bu Binding Site BS02

Receptor Information

>8ove Chain Bu (length=240) Species: 5702 (Trypanosoma brucei brucei)

VKVVKNKAYYKRFQVKYRRRREGKTDYHARRRMVLQDKTKFGTPKYRLVV
RITNRDVIAQIVHAKVVGDEVVMAAYSHELPLFGIEHGLTNYAAAYATGL
LVARRMLAKLGLAEKFVGVKEVDGSYAAVRTKDDDQGDDESRFPFKAILD
VGLARTTTGARVFGVLKGAVDGGLAVPHRPNRFPGYNKESDALNAKVHRD
RIFGRHVADYLKQVREEASSDMEGMYKRAHAAIRADPTKR

Ligand information

>8ove Chain BB (length=1109)

caacugcagaccguacucaucaccgcaucagguccccaagcaucgaaugc
ucugguacauagagaaagguacacucagggaagucggcaaaauagaugcg
uaaguucgcaagaagcauuggcucugagggcaauugccaacucagaacug
cuuacggcggggaauccaacuguauaauuaaaacauagguuugugaugca
uccaagugguguaugucgcaaacugauuucugcccagugcucugaauguc
aacgugacgagauucaccgacgcgcggguaaacggcgggaguaacuauga
cucucuuaagguagccaaaugccucgucuuccaauuagagacgcgcauga
auggauuaaugagauucccucugucccgaguuacuaucuagcgaaacgac
agucaagggaacggacuugaagggcuaagcggggaaagaagacccuguug
aguuugacuccagucuggcucugugcggcgacuuacguugcuucacuuau
cgaaugaagagaccaaugguuguuuugcgcagucuucgggcugugcgccg
ucuaggcugacgcgugcggaacucugcgccaacgggguggaauucuccgg
agucuuguuucgagacaucugccagauggggaguuuggcuggggcggcau
aucuguuacacgacaacgcagguguccuaaggcgagcucagugggaucuc
acguggaacacaagcguaaaugcuugcuugauuaacgauuuccaguacga
aucgagacugcgaaagcaaggccagcgauccuuugcgaagaacaggaaau
augaacaaaucauaccagaggugucagaauaauuaccacagggauaacug
gcuuguggcggccaagcguucauagcgacgucgcuuuuugauccuucgau
gucggcucuuccuaaccuagcgccgcagaagacgcuaaggguuggauugu
ucacccacugacagggaacgugagcuggguuuagaccgucgugagacagg
uugguuuuacccuacuuggcuggagauugcgaguaaaagauuauccgcgc
aaagcggaggcaguccguuuuuuucugggucugccguuggguggcaugug
cuuggcgcu

......................<<......>>......<<<<<...>>>>
>........<<<<....<<<<.<<<<.<<.........<.....<<<<<.
....<..>......>>>>><<.<<<<<<<<<<<>>>>>..>>>>>>.>>.
.........<<<<<<<<<..<<<.........>>>..<<<<<<<....<<
<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<.<..<<<.
...<<<<......>>>>....>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<.......>>>>>.....>..
...>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>..........
..<<<<<........>>>>>..<<<<<<<..<<<.<<......<<<<.<<
<<<<..<<<<<.<<<<<<<...<<<.<<<<<<....>>>>>>.>>>...<
<<<<.....<<<<....<<<......<<<<<<<......>>>>>>>>>>.
....<..<...>..>...................<<<.<<..>>.>>>..
.>>>>...>>>>>.......>>>>>>>..>>>>>...<<<.....>>>..
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<..>>>
>>.....<<......>>....>>>>>>>...<<...<<<<<.......>>
>>>..>>.<<<....>>>.>>.........<<<<<..............<
<<<.......>>>>.>>>>>..........>>>>..>>>>>>.......<
<.<.<.<<<<<<...<<.......>>...>>>>>>...>>.>.>......
.<<<<<.<<<<<<..<<<<<<<.<......>.>>>>..>>>.<<<.....
....>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>
>>....>>..>>>.>>>>>>>.......................<<<<..
...>>>>...<<<...........>>>..........<..<.<.......
>.>.>....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8ove A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): V4 K8 Y12 F16 Q17 K19 R23 T28 R35 M36 K48 H181 R182 R185
• Binding residue (residue number reindexed from 1): V1 K5 Y9 F13 Q14 K16 R20 T25 R32 M33 K45 H178 R179 R182

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0008097 5S rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation
• GO:0042255 ribosome assembly

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ove, PDBe:8ove, PDBj:8ove
• PDBsum: 8ove
• PubMed: 37985661
• UniProt: Q38CY8