Structure of PDB 4v9h Chain BT Binding Site BS02

Receptor Information

>4v9h Chain BT (length=137) Species: 300852 (Thermus thermophilus HB8)

MNRGALIKLVESRYVRTDLPEFRPGDTVRVSYKVKEGNRTRIQDFEGIVI
RIRRNGFNTTFTVRKVSYGVGVERIFPLHSPLIQKIDIVQRGRARRAKLY
FIRNLSDREIRRKLRADRKRIDQDRAAERAAKEEAQK

Ligand information

>4v9h Chain BA (length=2859)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
uugcgcggggggaaccuggggaacugaaacaucucaguacccagaggaga
ggaaagagaaaucgacucccugaguagcggcgagcgaaaggggaccagcc
uaaaccguccggcuuguccgggcggggucgggggcccucggccgaauccc
cagccuagccgaagcuguugggaagcagcgccagagagggugaaagcccc
guaggcgaaagguggggggauaggugaggguacccgaguaccccgugguu
cguggagccaugggggaaucugggcggaccaccgccuaaggcuaaguacu
ccgggugaccgauagcgcaccaguaccgugagggaaaggugaaaagaacc
ccgggaggggagugaaauagagccugaaaccgugggcuuacaagcaguca
cggccccaagggguuguggcgugccuauugaagcaugagccggcgacuca
cggucgugggcgagcuuaagccguugaggcggaggcguagggaaaccgag
uccgaacagggcgcguccgcggccguggacccgaaaccgggcgagcuagc
ccuggccagggugaagcuggggugagacccaguggaggcccgaaccggug
ggggaugcaaaccccucggaugagcuggggcuaggagugaaaagcuaacc
gagcccggagauagcugguucuccccgaaaugacuuuagggucagccuca
ggcgcugacuggggccuguagagcacugauagggcuagggggcccaccag
ccuaccaaacccugucaaacuccgaagggucccagguggagccugggagu
gagggcgcgagcgauaacguccgcguccgagcgcgggaacaaccgagacc
gccagcuaaggcccccaagucugggcuaagugguaaaggauguggcgccg
cgaagacagccaggagguggcuuagaagcagccauccuuuaaagagugcg
uaauagcucacuggucgaguggcgccgcgccgaaaaugaucggggcuuaa
gcccagcgccgaagcugcgggucugggggaugaccccaggcgguagggga
gcguucccgaugccgaugaaggccgacccgcgagggcggcuggagguaag
ggaagugcgaaugccggcaugaguaacgauaaagagggugagaaucccuc
ucgccguaagcccaaggguuccuacgcaauggucgucagcguaggguuag
gcgggaccuaaggugaagccgaaaggcguagccgaagggcagccgguuaa
uauuccggcccuucccgcaggugcgauggggggacgcucuaggcuagggg
gaccggagccauggacgagcccggccagaagcgcagggugggagguaggc
aaauccgccucccaacaagcucugcgugguggggaagcccggugacaacc
ccccgaagccagggagccaagaaaagccucuaagcacaaccugcgggaac
ccguaccgcaaaccgacacaggugggcgggugcaagagcacucaggcgcg
cgggagaacccucgccaaggaacucugcaaguuggccccguaacuucggg
agaaggggugcucccuggggugaugagccccggggagccgcagugaacag
gcucuggcgacuguuuaccaaaaacacagcucucugcgaacucguaagag
gagguauagggagcgacgcuugcccggugccggaaggucaaggggagggg
ugcaagccccgaaccgaagccccggugaacggcggcguaacuauaacggu
ccuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaaaag
cguaacgaccggagcgcugucucggcgagggacccggugaaauugaacug
gccgugaagaugcggccuacccguggcaggacgaaaagaccccguggagc
uuuacugcagccugguguuggcucuuggucgcgccugcguaggauaggug
ggagccugugaacccccgccuccgggugggggggaggcgccggugaaaua
ccacccuggcgcggcugggggccuaacccucggauggggggacagcgcuu
ggcgggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgc
ccaaagguccccucaggcgggacggaaauccgccggagagcgcaagggua
gaagggggccugacugcgaggccugcaagccgagcaggggcgaaagccgg
gccuagugaaccgguggucccguguggaagggccaucgaucaacggauaa
aaguuaccccggggauaacaggcugaucucccccgagcguccacagcggc
ggggagguuuggcaccucgaugucggcucgucgcauccuggggcugaaga
aggucccaaggguugggcuguucgcccauuaaagcggcacgcgagcuggg
uucagaacgucgugagacaguucggucucuauccgccacgggcgcaggag
gcuugaggggggcucuuccuaguacgagaggaccggaagggacgcaccuc
ugguuucccagcugucccuccaggggcauaagcuggguagccaugugcgg
aagggauaaccgcugaaagcaucuaagcgggaagcccgccccaagaugag
gccucccacggcgaagccgguaaggacccgggaagaccacccgguggaug
ggccggggguguaagcgccgcgaggcguugagccgaccggucccaaucgu
ccgaggucu

.<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<<
<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>>
>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<<
..>>>>>>.>>...<<<<<<.....<<....>>......>>>>>.>....
.....<<....>>...<<<<<.....<<.....>>....>>>>>...<<<
....<<<<<<<<.....>>>>>>>>.....<<<<<<<<...<<...<<<<
<.<<<<.<<<...<<<<<......>>>>>((......<<<.....))>>>
...>>>...>>>>..>>>>>..>>..>>>>>.>>>.....<<<<<<<<<.
......>>>>>>>>>......<<<<<..((((>>>>>..>>>......>>
.>>>>>>.>>...............<<....>>....>>.........<<
<<....>>>>..................>>>>>>>>>>>.......<<<<
<<<<<<<..>>>>>>>>>>>...<............>.<<<<<<....<<
<<<<<<<<<<...<<<...<<<.....>>>...>>>...<<....>>..<
<<<.....>>>>...>>>>>>>>>>>>..<<.<...<<<<<<.....<<<
<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<..<
<<<........>>>>.>>>....>>>>>>>>>...<<<.....>>>....
..>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<<<<<
<<<.....<<<<<<<<<...<<<...<<<<<<<<...<<<<<........
>>>.>>...>>>>>>>>..>>.>...>>>>>>>>>.....>>>>>>>.>>
..<<<<<<<.<<......>>.>>>>>>>.....<<<......>>>...<<
<<.<<<...<<<(((.....<<<<<<...........<<..<<<<<<<<<
........<<<<<.<<<..<<<.......>>>...>>>.........<<.
.....>>...>>>>>....>>>>>>>>>.>>..........)))......
>>>>>>.>>>...>>>>>>><<<<<<<<.....>>>>>>>>....>>>>>
>..<<<<<...<<<......<<<<<.<<<....>>>>>>>>...>>>..>
>>>>........>>>>>><......<<.....<<<<<<.......>>>>>
><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....(
(<<<<.<<...<<<...<<<....>>>...>>>...>>...<<<<<....
....>>>>>))<<<<<<<<<<<<<...<<<<....<<<<<.<<<..<<<<
<.<<<<.<<..[[[[...>>>>>><<<...<<<<<<<<<<<<<<<<.<<.
....>>>>>>>>>.....>>>>>>>>>..>>><...<<<.>>>.....>>
>>>>...>>>..>>>>>.........>>>>...>>...>>>>>>>>>>>>
>>>..>>.....<<......>>>>>>>.<<<<<....>>>>>..>>>>..
<<<<....<<<<<<<<.<<.........<.....<<<<<...........
....>>>>><<<<<<<<<<<<.....>>>>>>>>>>>>............
<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>>
.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<...<<...<<<<
<....>>>>>...>>...>>>>>>>>..>>>>><<<<<.......>>>.>
>........<...........<<<<<<.....>>>>>>.....>....>>
>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<
<<<<........>>>>>..<<<<<<<<.<<<.........<<<<.<<<<<
<..<<<<<.<<<.<<<<<.<<<<<<<<<<<<<<<<...........<<<<
<..<<<......<<<<<<<<....>>>>>>>>...>>>............
>>>>>..>>>>>>>>>>>>>>>>...<<<<<....>>>>>....>>>>>.
>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..
<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>.
...>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>
>))).......<<<<<<<.<<..))))..>>>>>>>>>............
.>>>>..>>>>>>.......<<<<...<<<<<<...<<.......>>...
>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.....
.>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>
....<<<<<<<....>>>.>>>>........>>>>>>>>>>>....<<<<
<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...
<<<<...<<<<<<<<<<<]]]]>>>>>>...>>>>>...>>>>.>>>>>.
..<<<....<<<<<...........>>>>>....>>>.>>>>.......>
>>>>>>...<.......>.....<<<<<<<<<.......>>>>>....<<
<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>
>>....>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v9h Elongation factor G bound to the ribosome in an intermediate state of translocation.
• Resolution: 2.857 Å
• Binding residue (original residue number in PDB): N2 R3 A5 R23 R51 R53 R54 N55 N58 T60 R93 R95 R96 A97 K98 Y100 F101 K113 K119
• Binding residue (residue number reindexed from 1): N2 R3 A5 R23 R51 R53 R54 N55 N58 T60 R93 R95 R96 A97 K98 Y100 F101 K113 K119

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v9h, PDBe:4v9h, PDBj:4v9h
• PDBsum: 4v9h
• PubMed: 23812720
• UniProt: P60490|RL19_THET8 Large ribosomal subunit protein bL19 (Gene Name=rplS)