Structure of PDB 8ova Chain BQ Binding Site BS02

Receptor Information

>8ova Chain BQ (length=203) Species: 5702 (Trypanosoma brucei brucei)

GSFMYLNELWKKKSSDVMRFIQRVRAWEFRHQHTIVRLRRPTRPEKARMV
GYKTKQGYVVFRVRVRRGGRKRPVHKGITYGKPNTAGVLGRKLNKNNRVV
AEQRLGKKYGNLRVLNSYWVNADSTFLWYEVVAVDPMHRAIRRDPRINWI
VNAVHKHREQRGLTSAGRKHRGLRQKGHKASKLRPSRRAAWRRNNRMVFL
RKR

Ligand information

>8ova Chain BB (length=1119)

cccaacugcagaccguacucaucaccgcaucagguccccaagcaucgaau
gcucugguacauagagaaagguacacucagggaagucggcaaaauagaug
cguaaguucgcaagaagcauuggcucugagggcaaaaauugccaacucag
aacugcuuacggcggggaauccaacuguauaauuaaaacauagguuugug
augcauccaagugguguaugucgcaaacugauuucugcccagugcucugu
gucaacgugacgagauucaccgacgcgcggguaaacggcgggaguaacua
ugacucucuuaagguagccaaaugccucgucuuccaauuagagacgcgca
ugaauggauuaaugagauucccucugucccgaguuacuaucuagcgaaac
gacagucaagggaacggacuugaagggcuaagcggggaaagaagacccug
uugaguuugacuccagucuggcucugugcggcgacuuacguugcuucacu
uaucgaaugaagagaccaaugguuguuuugcgcagucuucgggcugugcg
ccgucuaggcugacgcgugcggaacuacugcgccaaacggggugguucuc
cggagucuuguuucgagacaucugccagauggggaguuuggcuggggcgg
cauaucuguuacacgacaacgcagguguccuaaggcgagcucagugggaa
cagaaaucucacguggaacacaagcguaaaugcuugcuugauuaacgauu
uccaguacgaaucgagacugcgaaagcaaggccagcgauccuuugcgaag
aacaggaaauaugaacaaaucauaccagaggugucagaauaauuaccaca
gggauaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuug
auccuucgaugucggcucuuccuaaccuagcgccgcagaagacgcuaagg
guuggauuguucacccacugacagggaacgugagcuggguuuagaccguc
gugagacagguugguuuuacccuacuuggcuggagauugcgaguaaaaga
uuauccgcgcaaagcggaggcaguccguuuuuuucugggucugccguugg
guggcaugugcuuggcgcu

........................<<......>>......<<<<<...>>
>>>........<<<<....<<<<.<<<<.<<.........<.....<<<<
<.....<..>......>>>>><<.<<<<<<<<<<<...>>>>>..>>>>>
>.>>..........<<<<<<<<<..<<<.........>>>..<<<<<<<.
...<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<.<<
<<....<<<<......>>>>....>>>>>>>>..>>>>><<<<<<.....
..>>>>>>........<...........<<<<<.......>>>>>.....
>.....>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>.......
.....<<<<<........>>>>>..<<<<<<<..<<<.<<......<<<<
.<<<<<<..<<<<<.<<<<<<<...<<<.<<<<<<....>>>>>>.>>>.
..<<<<<.....<<<<....<<<......<<<<<<<......>>>>>>>>
>>...<.<..<...>..>>....................<<<.<..>.>>
>...>>>>...>>>>>.......>>>>>>>..>>>>>...<<<.....>>
>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<.
......>>>>>>.....<<......>>....>>>>>>>...<<...<<<<
<.......>>>>>..>>.<<<....>>>.>>.........<<<<<.....
.........<<<<.......>>>>.>>>>>..........>>>>..>>>>
>>.......<<.<.<.<<<<<<...<<.......>>...>>>>>>...>>
.>.>.......<<<<<.<<<<<<..<<<<<<<.<......>.>>>>..>>
>.<<<.........>>>.....>>>>>..>...>>>.>>....<<<<<<<
....>>>.>>>>....>>..>>>.>>>>>>>...................
....<<<<.....>>>>...<<<...........>>>..........<..
<.<.......>.>.>....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8ova A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.
• Resolution: 2.47 Å
• Binding residue (original residue number in PDB): R48 R84 K89 P91 H93 K94 L107
• Binding residue (residue number reindexed from 1): R30 R66 K71 P73 H75 K76 L89

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ova, PDBe:8ova, PDBj:8ova
• PDBsum: 8ova
• PubMed: 37985661
• UniProt: Q38CY7