Structure of PDB 8ove Chain BI Binding Site BS02

Receptor Information

>8ove Chain BI (length=191) Species: 5702 (Trypanosoma brucei brucei)

GVDLTGVQKKKRVVRHHTYSTNPYIKLLIKLYKFLGKRTNSPFNKLIHKR
LLKSRNNRAPISLSRIAVCMRRRTVWLKKGKKSPIAVIVGDVLDDVRMTR
IPALRICALRFSKSARERITGAGGECLTFDQLAMMAPTGKNTMLLRGRKS
GRESVKHFGAAGVPGSHAKPHVSSRGKERQRSSKRRHAFRH

Ligand information

>8ove Chain BB (length=1109)

caacugcagaccguacucaucaccgcaucagguccccaagcaucgaaugc
ucugguacauagagaaagguacacucagggaagucggcaaaauagaugcg
uaaguucgcaagaagcauuggcucugagggcaauugccaacucagaacug
cuuacggcggggaauccaacuguauaauuaaaacauagguuugugaugca
uccaagugguguaugucgcaaacugauuucugcccagugcucugaauguc
aacgugacgagauucaccgacgcgcggguaaacggcgggaguaacuauga
cucucuuaagguagccaaaugccucgucuuccaauuagagacgcgcauga
auggauuaaugagauucccucugucccgaguuacuaucuagcgaaacgac
agucaagggaacggacuugaagggcuaagcggggaaagaagacccuguug
aguuugacuccagucuggcucugugcggcgacuuacguugcuucacuuau
cgaaugaagagaccaaugguuguuuugcgcagucuucgggcugugcgccg
ucuaggcugacgcgugcggaacucugcgccaacgggguggaauucuccgg
agucuuguuucgagacaucugccagauggggaguuuggcuggggcggcau
aucuguuacacgacaacgcagguguccuaaggcgagcucagugggaucuc
acguggaacacaagcguaaaugcuugcuugauuaacgauuuccaguacga
aucgagacugcgaaagcaaggccagcgauccuuugcgaagaacaggaaau
augaacaaaucauaccagaggugucagaauaauuaccacagggauaacug
gcuuguggcggccaagcguucauagcgacgucgcuuuuugauccuucgau
gucggcucuuccuaaccuagcgccgcagaagacgcuaaggguuggauugu
ucacccacugacagggaacgugagcuggguuuagaccgucgugagacagg
uugguuuuacccuacuuggcuggagauugcgaguaaaagauuauccgcgc
aaagcggaggcaguccguuuuuuucugggucugccguuggguggcaugug
cuuggcgcu

......................<<......>>......<<<<<...>>>>
>........<<<<....<<<<.<<<<.<<.........<.....<<<<<.
....<..>......>>>>><<.<<<<<<<<<<<>>>>>..>>>>>>.>>.
.........<<<<<<<<<..<<<.........>>>..<<<<<<<....<<
<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<.<..<<<.
...<<<<......>>>>....>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<.......>>>>>.....>..
...>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>..........
..<<<<<........>>>>>..<<<<<<<..<<<.<<......<<<<.<<
<<<<..<<<<<.<<<<<<<...<<<.<<<<<<....>>>>>>.>>>...<
<<<<.....<<<<....<<<......<<<<<<<......>>>>>>>>>>.
....<..<...>..>...................<<<.<<..>>.>>>..
.>>>>...>>>>>.......>>>>>>>..>>>>>...<<<.....>>>..
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<..>>>
>>.....<<......>>....>>>>>>>...<<...<<<<<.......>>
>>>..>>.<<<....>>>.>>.........<<<<<..............<
<<<.......>>>>.>>>>>..........>>>>..>>>>>>.......<
<.<.<.<<<<<<...<<.......>>...>>>>>>...>>.>.>......
.<<<<<.<<<<<<..<<<<<<<.<......>.>>>>..>>>.<<<.....
....>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>
>>....>>..>>>.>>>>>>>.......................<<<<..
...>>>>...<<<...........>>>..........<..<.<.......
>.>.>....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8ove A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): R182 S183 S184 K185 H192
• Binding residue (residue number reindexed from 1): R181 S182 S183 K184 H191

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ove, PDBe:8ove, PDBj:8ove
• PDBsum: 8ove
• PubMed: 37985661
• UniProt: P50885|RL18_TRYB2 Large ribosomal subunit protein eL18 (Gene Name=RPL18-A)