Structure of PDB 7zrs Chain BI Binding Site BS02

Receptor Information

>7zrs Chain BI (length=218) Species: 4932 (Saccharomyces cerevisiae)

ARRPARCYRYQKNKPYPKSRYNRAVPDSKIRIYDLGKKKATVDEFPLCVH
LVSNELEQLSSEALEAARICANKYMTTVSGRDAFHLRVRVHPFHVLRINK
MLSCAGADRLQQGMRGAWGKPHGLAARVDIGQIIFSVRTKDSNKDVVVEG
LRRARYKFPGQQKIILSKKWGFTNLDRPEYLKKREAGEVKDDGAFVKFLS
KKGSLENNIREFPEYFAA

Ligand information

>7zrs Chain 5 (length=3185)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacugggccagcaucaguuuugguggcaggauaaauccauaggaaug
uagcuugccucgguaaguauuauagccugugggaauacugccagcuggga
cugaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccg
cccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugg
guguaaaacccauacgcguaaugaaagugaacguagguuggggccucgca
agaggugcacaaucgaccgauccugaugucuucggauggauuugaguaag
agcauagcuguugggacccgaaagauggugaacuaugccugaauagggug
aagccagaggaaacucugguggaggcucguagcgguucugacgugcaaau
cgaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagu
agcugguuccugccgaaguuucccucaggauagcagaagcucguaucagu
uuuaugagguaaagcgaaugauuagagguuccggggucgaaaugaccuug
accuauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaa
cguggacauuugaaugaagagcuuuuagugggccauuuuugguaagcaga
acuggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacac
gcucaucagacaccacaaaagguguuaguucaucuagacagccggacggu
ggccauggaagucggaauccgcuaaggaguguguaacaacucaccggccg
aaugaacuagcccugaaaauggauggcgcucaagcguguuaccuauacuc
uaccgucaggguugauaugaugcccugacgaguaggcaggcguggagguc
agugacgaagccuagaccguaaggucgggucgaacggccucuagugcaga
ucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaagug
gggaaagguuccacgucaacagcaguuggacguggguuagucgauccuaa
gagauggggaagcuccguuucaaaggccugauuuuaugcaggccaccauc
gaaagggaauccgguuaagauuccggaaccuggauauggauucuucacgg
uaacguaacugaauguggagacgucggcgcgagcccugggaggaguuauc
uuuucuucuuaacagcuuaucaccccggaauugguuuauccggagauggg
gucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugu
gacggcccgugaaaauccacaggaaggaauaguuuucaugccaggucgua
cugauaaccgcagcaggucuccaaggugaacagccucuaguugauagaau
aauguagauaagggaagucggcaaaauagauccguaacuucgggauaagg
auuggcucuaagggucggguacgaucaacuuagaacugguacggacaagg
ggaaucugacugucuaauuaaaacauagcauugcgauggucagaaaguga
uguugacgcaaugugauuucugcccagugcucugaaugucaaagugaaga
aauucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaag
guagccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaac
gagauucccacugucccuaucuacuaucuagcgaaaccacagccaaggga
acgggcuuggcagaaucagcggggaaagaagacccuguugagcuugacuc
uaguuugacauugugaagagacauagagggcuaccuuuauaguuucuuua
cuuauucaaugaagcggagcuggaauucauuuuccacguucuagcauuca
aggucccauucggggcugauccggguugaagacauugucagguggggagu
uuggcuggggcggcacaucuguuaaacgauaacgcagauguccuaagggg
ggcucauggagaacagaaaucuccaguagaacaaaaggguaaaagccccc
uugauuuugauuuucagugugaauacaaaccaugaaaguguggccuaucg
auccuuuagucccucggaauuugaggcuagaggugccagaaaaguuacca
cagggauaacuggcuuguggcagucaagcguucauagcgacauugcuuuu
ugauucuucgaugucggcucuuccuaucauaccgaagcagaauucgguaa
gcguuggauuguucacccacuaauagggaacgugagcuggguuuagaccg
ucgugagacagguuaguuuuacccuacugaugaauguuaccgcaauagua
auugaacuuaguacgagaggaacaguucauucggauaauugguuuuugcg
gcugucugaucaggcauugccgcgaagcuaccauccgcuggauuauggcu
gaacgccucuaagucagaauccaugcuagaacgcggugauuucuuugcuc
cacacaauauagauggauacgaauaaggcguccuuguggcgucgcugaac
cauagcaggcuagcaacggugcacuuggcggaaaggccuugggugcuugc
uggcgaauugcaaugucauuuugcguggggauaaaucauuuguauacgac
uuagauguacaacgggguauuguaagcaguagaguagccuuguuguuacg
aucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.................
.........<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<<....
..<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>
>>>........<<<....>>>.....>>>>>>>.....>>>>>.>>>>>.
.<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<
<<.....>>>>...>>>...<<....>>.......<<<<<..<<<<<...
.>>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>...
.>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>
>>>>>>>....>>>>>>>>>...<<.......>>......>>>>>>...>
..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<
<<<<<<.....<<<....<<<..<.<<<<...<<<<<.......>>>>>.
>>>>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>
>>.>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>
>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<
<<.....<<..........<<<.<<<<<<<<<<.......<<<<<.<<..
..<<.........>>....>>.........<<......>>...>>>>>..
.>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>
>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<
.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>....
.>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<
<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...
<<....<<<....>>>........<<<.<<........>.>>>>....>>
....>>...<<<<<........>>>>>))<<<<.<<<<<.<.....<<<.
...>>>.......<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..{{{
..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>
>>.....>>>>>....><<<<<<<......>>>>>>>>>>>.>.>>>>>>
>.>>>>.........>>>>>>..........>.>..>>>>>>>>>>>>..
>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<..
...<<<<<<<.<<.........<.....<<<<<....<<..>>.....>>
>>><<.<<<<<<<<<<<....>>>>>..>>>>>>.>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>
>......>>>>>>>...<<..<<<..<<<<<<.....<<.....<<<<..
..>>>>.....>>..>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<.......>>>>>.....>.....>>....>
>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<...
.....>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<
<.<<<<<<<<..<<<<<<<<<<<<<<<<<....>>>>>>>.>>>>>>>>>
>...<<<<<.....<<<<..<<<<<......>>>>>..............
.<<<<<<....>>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>
...<<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<
<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>
>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))....
...((((((..((((.>>>>>>))))))))))..........]]]]..}}
]]]].......<<.<.<.<<<<<<...<<.......>>...>>>>>>...
>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>.
..>.<<<.........>>>.....>>>>>..>...>>>.>>....<<<<<
<<....>>>.>>>>....}}..}}}.]]]]]....<<<<<<<<..<<<<.
<.<<<<<<...............>>>>>>.><<<<<...<<<<....<<<
<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<
...........>>>>>...>>>>.>>>>....>>>>>>>>.......<<<
<<<.<<<.<<....<<..........<<<<<<.....>>>>>>......>
>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>
>>>>.............>>.>>>.>>>>>>..<<<<<.<...........
..>.....<<<<<<<<<.<<<....<<<<....<<<<<......>>>>>.
..>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zrs Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 4.8 Å
• Binding residue (original residue number in PDB): R3 R4 A6 R7 K13 N14 K15 Y22 R24 Y34 D35 K39 K40 E63 A64 A67 K74 R90 H92 F94 R98 N100 G107 A108 D109 R110 G114 M115 R116 W119 G120 Y157 K158 P160 G194 F196 K198
• Binding residue (residue number reindexed from 1): R2 R3 A5 R6 K12 N13 K14 Y21 R23 Y33 D34 K38 K39 E62 A63 A66 K73 R89 H91 F93 R97 N99 G106 A107 D108 R109 G113 M114 R115 W118 G119 Y156 K157 P159 G193 F195 K197

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006415 translational termination

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zrs, PDBe:7zrs, PDBj:7zrs
• PDBsum: 7zrs
• PubMed: 36801861
• UniProt: P41805|RL10_YEAST Large ribosomal subunit protein uL16 (Gene Name=RPL10)