Structure of PDB 7zrs Chain BD Binding Site BS02

Receptor Information

>7zrs Chain BD (length=294) Species: 4932 (Saccharomyces cerevisiae)

QKDAKSSAYSSRFQTPFRRRREGKTDYYQRKRLVTQHKAKYNTPKYRLVV
RFTNKDIICQIISSTITGDVVLAAAYSHELPRYGITHGLTNWAAAYATGL
LIARRTLQKLGLDETYKGVEEVEGEYELTEAVEDGPRPFKVFLDIGLQRT
TTGARVFGALKGASDGGLYVPHSENRFPGWDFETEEIDPELLRSYIFGGH
VSQYMEELADDDEERFSELFKGYLADDIDADSLEDIYTSAHEAIRADPAF
KPTEKKFTKEQYAAESKKYRQTKLSKEERAARVAAKIAALAGQQ

Ligand information

>7zrs Chain 5 (length=3185)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacugggccagcaucaguuuugguggcaggauaaauccauaggaaug
uagcuugccucgguaaguauuauagccugugggaauacugccagcuggga
cugaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccg
cccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugg
guguaaaacccauacgcguaaugaaagugaacguagguuggggccucgca
agaggugcacaaucgaccgauccugaugucuucggauggauuugaguaag
agcauagcuguugggacccgaaagauggugaacuaugccugaauagggug
aagccagaggaaacucugguggaggcucguagcgguucugacgugcaaau
cgaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagu
agcugguuccugccgaaguuucccucaggauagcagaagcucguaucagu
uuuaugagguaaagcgaaugauuagagguuccggggucgaaaugaccuug
accuauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaa
cguggacauuugaaugaagagcuuuuagugggccauuuuugguaagcaga
acuggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacac
gcucaucagacaccacaaaagguguuaguucaucuagacagccggacggu
ggccauggaagucggaauccgcuaaggaguguguaacaacucaccggccg
aaugaacuagcccugaaaauggauggcgcucaagcguguuaccuauacuc
uaccgucaggguugauaugaugcccugacgaguaggcaggcguggagguc
agugacgaagccuagaccguaaggucgggucgaacggccucuagugcaga
ucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaagug
gggaaagguuccacgucaacagcaguuggacguggguuagucgauccuaa
gagauggggaagcuccguuucaaaggccugauuuuaugcaggccaccauc
gaaagggaauccgguuaagauuccggaaccuggauauggauucuucacgg
uaacguaacugaauguggagacgucggcgcgagcccugggaggaguuauc
uuuucuucuuaacagcuuaucaccccggaauugguuuauccggagauggg
gucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugu
gacggcccgugaaaauccacaggaaggaauaguuuucaugccaggucgua
cugauaaccgcagcaggucuccaaggugaacagccucuaguugauagaau
aauguagauaagggaagucggcaaaauagauccguaacuucgggauaagg
auuggcucuaagggucggguacgaucaacuuagaacugguacggacaagg
ggaaucugacugucuaauuaaaacauagcauugcgauggucagaaaguga
uguugacgcaaugugauuucugcccagugcucugaaugucaaagugaaga
aauucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaag
guagccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaac
gagauucccacugucccuaucuacuaucuagcgaaaccacagccaaggga
acgggcuuggcagaaucagcggggaaagaagacccuguugagcuugacuc
uaguuugacauugugaagagacauagagggcuaccuuuauaguuucuuua
cuuauucaaugaagcggagcuggaauucauuuuccacguucuagcauuca
aggucccauucggggcugauccggguugaagacauugucagguggggagu
uuggcuggggcggcacaucuguuaaacgauaacgcagauguccuaagggg
ggcucauggagaacagaaaucuccaguagaacaaaaggguaaaagccccc
uugauuuugauuuucagugugaauacaaaccaugaaaguguggccuaucg
auccuuuagucccucggaauuugaggcuagaggugccagaaaaguuacca
cagggauaacuggcuuguggcagucaagcguucauagcgacauugcuuuu
ugauucuucgaugucggcucuuccuaucauaccgaagcagaauucgguaa
gcguuggauuguucacccacuaauagggaacgugagcuggguuuagaccg
ucgugagacagguuaguuuuacccuacugaugaauguuaccgcaauagua
auugaacuuaguacgagaggaacaguucauucggauaauugguuuuugcg
gcugucugaucaggcauugccgcgaagcuaccauccgcuggauuauggcu
gaacgccucuaagucagaauccaugcuagaacgcggugauuucuuugcuc
cacacaauauagauggauacgaauaaggcguccuuguggcgucgcugaac
cauagcaggcuagcaacggugcacuuggcggaaaggccuugggugcuugc
uggcgaauugcaaugucauuuugcguggggauaaaucauuuguauacgac
uuagauguacaacgggguauuguaagcaguagaguagccuuguuguuacg
aucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.................
.........<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<<....
..<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>
>>>........<<<....>>>.....>>>>>>>.....>>>>>.>>>>>.
.<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<
<<.....>>>>...>>>...<<....>>.......<<<<<..<<<<<...
.>>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>...
.>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>
>>>>>>>....>>>>>>>>>...<<.......>>......>>>>>>...>
..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<
<<<<<<.....<<<....<<<..<.<<<<...<<<<<.......>>>>>.
>>>>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>
>>.>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>
>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<
<<.....<<..........<<<.<<<<<<<<<<.......<<<<<.<<..
..<<.........>>....>>.........<<......>>...>>>>>..
.>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>
>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<
.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>....
.>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<
<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...
<<....<<<....>>>........<<<.<<........>.>>>>....>>
....>>...<<<<<........>>>>>))<<<<.<<<<<.<.....<<<.
...>>>.......<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..{{{
..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>
>>.....>>>>>....><<<<<<<......>>>>>>>>>>>.>.>>>>>>
>.>>>>.........>>>>>>..........>.>..>>>>>>>>>>>>..
>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<..
...<<<<<<<.<<.........<.....<<<<<....<<..>>.....>>
>>><<.<<<<<<<<<<<....>>>>>..>>>>>>.>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>
>......>>>>>>>...<<..<<<..<<<<<<.....<<.....<<<<..
..>>>>.....>>..>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<.......>>>>>.....>.....>>....>
>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<...
.....>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<
<.<<<<<<<<..<<<<<<<<<<<<<<<<<....>>>>>>>.>>>>>>>>>
>...<<<<<.....<<<<..<<<<<......>>>>>..............
.<<<<<<....>>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>
...<<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<
<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>
>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))....
...((((((..((((.>>>>>>))))))))))..........]]]]..}}
]]]].......<<.<.<.<<<<<<...<<.......>>...>>>>>>...
>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>.
..>.<<<.........>>>.....>>>>>..>...>>>.>>....<<<<<
<<....>>>.>>>>....}}..}}}.]]]]]....<<<<<<<<..<<<<.
<.<<<<<<...............>>>>>>.><<<<<...<<<<....<<<
<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<
...........>>>>>...>>>>.>>>>....>>>>>>>>.......<<<
<<<.<<<.<<....<<..........<<<<<<.....>>>>>>......>
>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>
>>>>.............>>.>>>.>>>>>>..<<<<<.<...........
..>.....<<<<<<<<<.<<<....<<<<....<<<<<......>>>>>.
..>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zrs Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 4.8 Å
• Binding residue (original residue number in PDB): Y12 R15 F16 Q17 P19 R23 T28 R35 L36 K43 Y44 K48 L113 P139 R140 F142 F145 I148 L150 R152 T153 F160 H175 S176 N178 R179
• Binding residue (residue number reindexed from 1): Y9 R12 F13 Q14 P16 R20 T25 R32 L33 K40 Y41 K45 L110 P136 R137 F139 F142 I145 L147 R149 T150 F157 H172 S173 N175 R176

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zrs, PDBe:7zrs, PDBj:7zrs
• PDBsum: 7zrs
• PubMed: 36801861
• UniProt: P26321|RL5_YEAST Large ribosomal subunit protein uL18 (Gene Name=RPL5)