Structure of PDB 4v68 Chain B0 Binding Site BS02

Receptor Information

>4v68 Chain B0 (length=85) Species: 274 (Thermus thermophilus)

MAHKKGLGSTRNGRDSQAKRLGVKRYEGQVVRAGNILVRQRGTRFKPGKN
VGMGRDFTLFALVDGVVEFQDRGRLGRYVHVRPLA

Ligand information

>4v68 Chain BA (length=2852)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
uugcgcggggggaaccuggggaacugaaacaucucaguacccagaggaga
ggaaagagaaaucgacucccugaguagcggcgagcgaaaggggaccagcc
uaaaccguccggcuuguccgggcggggucgggggcccucggccgaauccc
cagccuagccgaagcuguugggaagcagcgccagagagggugaaagcccc
guaggcgaaagguggggggauaggugaggguacccgaguaccccgugguu
cguggagccaugggggaaucugggcggaccaccgccuaaggcuaaguacu
ccgggugaccgauagcgcaccaguaccgugagggaaaggugaaaagaacc
ccgggaggggagugaaauagagccugaaaccgugggcuuacaagcaguca
cggccccaagggguuguggcgugccuauugaagcaugagccggcgacuca
cggucgugggcgagcuuaagccguugaggcggaggcguagggaaaccgag
uccgaacagggcgcguccgcggccguggacccgaaaccgggcgagcuagc
ccuggccagggugaagcuggggugagacccaguggaggcccgaaccggug
ggggaugcaaaccccucggaugagcuggggcuaggagugaaaagcuaacc
gagcccggagauagcugguucuccccgaaaugacuuuagggucagccuca
ggcgcugacuggggccuguagagcacugauagggcuagggggcgccuacc
aaacccugucaaacuccgaagggucccagguggagccugggagugagggc
gcgagcgauaacguccgcguccgagcgcgggaacaaccgagaccgccagc
uaaggcccccaagucugggcuaagugguaaaggauguggcgccgcgaaga
cagccaggagguuggcuuagaagcagccauccuuuaaagagugcguaaua
gcucacuggucgaguggcgccgcgccgaaaaugaucggggcuuaagccca
gcgccgaagcugcgggucugggggaugaccccaggcgguaggggagcguu
cccgaugccgaugaaggccgacccgcgagggcggcuggagguaagggaag
ugcgaaugccggcaugaguaacgauaaagagggugagaaucccucucgcc
guaagcccaaggguuccuacgcaauggucgucagcguaggguuaggcggg
accuaaggugaagccgaaaggcguagccgaagggcagccgguuaauauuc
cggcccuucccgcaggugcgauggggggacgcucuaggcuagggggaccg
gagccauggacgagcccggccagaagcgcagggugggagguaggcaaauc
cgccucccaacaagcucugcgugguggggaagcccgcaaccccccgaagc
cagggagccaagaaaagccucuaagcacaaccugcgggaacccguaccgc
aaaccgacacaggugggcgggugcaagagcacucaggcgcgcgggagaac
ccucgccaaggaacucugcaaguuggccccguaacuucgggagaaggggu
gcucccuggggugaugagccccggggagccgcagugaacaggcucuggcg
acuguuuaccaaaaacacagcucucugcgaacucguaagaggagguauag
ggagcgacgcuugcccggugccggaaggucaaggggaggggugcaagccc
cgaaccgaagccccggugaacggcggccguaacuauaacgguccuaaggu
agcgaaauuccuugucggguaaguuccgaccugcacgaaaagcguaacga
ccggagcgcugucucggcgagggacccggugaaauugaacuggccgugaa
gaugcggccuacccguggcaggacgaaaagaccccguggagcuuuacugc
agccugguguuggcucuuggucgcgccugcguaggauaggugggagccug
ugaacccccgccuccgggugggggggaggcgccggugaaauccacccugg
cgcggcugggggccuaacccucggauggggggacagcgcuuggcgggcag
uuugacuggggcggucgccuccuaaaagguaacggaggcgcccaaagguc
cccucaggcgggacggaaauccgccggagagcgcaaggguagaagggggc
cugacugcgaggccugcaagccgagcaggggcgaaagccgggccuaguga
accgguggucccguguggaagggccaucgaucaacggauaaaaguuaccc
cggggauaacaggcugaucucccccgagcguccacagcggcggggagguu
uggcaccucgaugucggcucgucgcauccuggggcugaagaaggucccaa
ggguugggcuguucgcccauuaaagcggcacgcgagcuggguucagaacg
ucgugagacaguucggucucuauccgccacgggcgcaggaggcuugaggg
gggcucuuccuaguacgagaggaccggaagggacgcaccucugguuuccc
agcugucccuccaggggcauaagcuggguagccaugugcggaagggauaa
ccgcugaaagcaucuaagcgggaagcccgccccaagaugaggccucccac
ggcgucaagccgguaaggacccgggaagaccacccgguggaugggccggg
gguguaagcgccgcgaggcguugagccgaccggucccaaucguccgaggu
cu

<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<<
<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>>
>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<<
..>>>>>>.>>....<<<<<.....<<....>>......>>>>>......
.....<<....>>...<<<<<.....<<.....>>....>>>>>...<<<
....<<<<<<<<.....>>>>>>>>.....<<<<<<<<.<.<<...<<<.
<.<<<<.<<<...<<<<<......>>>>>((......<<<.....))>>>
...>>>...>>>>...>>>>..>>>.>>>>>.>>>.....<<<<<<<<<.
......>>>>>>>>>......<<<<<..((((>>>>>..>>>......>>
.>>>>>>.>>...............<<....>>....>>.........<<
<<....>>>>..................>>>>>>>>>>>.......<<<<
<<<<<<....>>>>>>>>>>...<............>.<<<<<<....<<
<<<<<<<<<<...<<<...<<<.....>>>...>>>...<<....>>..<
<<<.....>>>>...>>>>>>>>>>>>..<<.<...<<<<<<.....<<<
<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<..<
<<<........>>>>.>>>....>>>>>>>>>...<<<.....>>>....
..>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<<<<<
<<<.....<<<<<<<<<...<<<...<<<<<<<<.....<<....>>...
...>>>>>>>>..>>.>...>>>>>>>>>.....>>>>>>>.>>..<<<<
<<<.<<......>>.>>>>>>>.....<<<......>>>...<<<<.<<<
...<<<(((.....<<<<<<...........<<..<<<<<<<<<......
...<<<<.<<.....................>>.........<.......
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>.>>>...>>>>>>><<<<<<<.......>>>>>>>....>>>>>>..<<
<<<...<<<......<<<<<.<<<....>>>>>>>>...>>>..>>>>>.
.......>>>>>><......<<.....<<<<<<.......>>>>>><<<<
....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<
.<<...<<<...<<<....>>>...>>>...>>...<<<<<........>
>>>>))<<<<<<<<<<<<<...<<<<....<<<<<.<<<..<<<<<.<<<
<.<<..[[[[...>>>>>><<<...<<<<<<<<<<<<<<<<.<<.....>
>>>>>>>>.....>>>>>>>>>..>>>.............>>>>>...>>
>..>>>>>.........>>>>...>>...>>>>>>>>>>>>>>>..>>..
...<<......>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<
<<<<<<<.<<.........<.....<<<<<.....<..>......>>>>>
<<<<<<<<<<<<.....>>>>>>>>>>>>............<<<<<<<<<
..<<<.........>>>..<<<<<<<.....<<<....>>>.......>>
>>>>>.<<<<<.<<<..<<<<<<<..<<<...<<...<<<<<....>>>>
>...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>.......
.<...........<<<<<<.....>>>>>>.....>....>>>>>..>>.
>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<....
....>>>>>..<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<
.<<<.<<<<<.<<<<<<<<<<<<<<<<...........<<<<<<.<<<<.
....<<<<<<<<....>>>>>>>>..>>>>..........>>>>>>..>>
>>>>>>>>>>>>>>...<<<<<....>>>>>....>>>>>.>>>..>>>>
>..<<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<
<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>
>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>))).....
..<<<<<<<.<<..))))..>>>>>>>>>.............>>>>..>>
>>>>.......<<<<.<.<<<<<<<..<<.......>>>..>>>>>>...
>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..
>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<
<<....>>>.>>>>........>>>>>>>>>>>....<<<<<<<...<<<
<..<<<<<<<...............>>>>>>>.<<<<<...<<<<...<<
<<<<<<<<<]]]]>>>>>>...>>>>>...>>>>.>>>>>...<<<....
<<<<<...........>>>>>....>>>.>>>>.......>>>>>>>...
<<.......>>.....<<<<<<<<<.......>>>>>....<<<<<<<<.
<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>....>
>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v68 GTPase activation of elongation factor EF-Tu by the ribosome during decoding
• Resolution: 6.4 Å
• Binding residue (original residue number in PDB): M1 A2 N12 R14 S16 Q17 A18 K19 R20 K24 Y26 E27 Q29 R32 A33 G34 N35 I36 R39 R41 G42 T43 F45 G54 R55 F57 F60 F69 R77
• Binding residue (residue number reindexed from 1): M1 A2 N12 R14 S16 Q17 A18 K19 R20 K24 Y26 E27 Q29 R32 A33 G34 N35 I36 R39 R41 G42 T43 F45 G54 R55 F57 F60 F69 R77

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v68, PDBe:4v68, PDBj:4v68
• PDBsum: 4v68
• PubMed: 19229291
• UniProt: P60493|RL27_THET8 Large ribosomal subunit protein bL27 (Gene Name=rpmA)