Structure of PDB 7lni Chain B Binding Site BS02

Receptor Information

>7lni Chain B (length=551) Species: 272563 (Clostridioides difficile 630)

SGIYYTPKIIVDYIVKKTLKNHDIIKNPYPRILDISCGCGNFLLEVYDIL
YDLFEENIYELKKKYDENYWTVDNIHRHILNYCIYGADIDEKAISILKDS
LTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFDYIVGNPPYIGHKKLEK
KYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVITPRYFLESL
SGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETF
YNKIQEKCKYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCW
IKSKNINKYIVDKSEYRLIYSNDIDNENTNKRILDEIIGLYKTKLENRRE
CKSGIRKWYELQWGREKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVY
SFFIKEEYLDKFSYEYLVGILNSSVYDKYFKITAKKMSKNIYDYYPNKVM
KIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLG
I

Ligand information

>7lni Chain G (length=14)

atgggactttttga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7lni Clostridioides difficile specific DNA adenine methyltransferase CamA squeezes and flips adenine out of DNA helix.
• Resolution: 2.68 Å
• Binding residue (original residue number in PDB): H171 K172 K179 D192 K193 I349 G351 D353 K378 K380 R425 Q438 W439 Y455 K456 K515
• Binding residue (residue number reindexed from 1): H145 K146 K153 D166 K167 I323 G325 D327 K352 K354 R399 Q412 W413 Y429 K430 K489

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0032259 methylation

External links

• PDB: RCSB:7lni, PDBe:7lni, PDBj:7lni
• PDBsum: 7lni
• PubMed: 34103525
• UniProt: Q183J3