Structure of PDB 4yye Chain B Binding Site BS02

Receptor Information
>4yye Chain B (length=417) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
ATMTSMVSQRQDLFMTDPLSPGSMFFLPNGAKIFNKLIEFMKLQQKFKFG
FNEVVTPLIYKKTLWEKSGHWENYADDMFKVETTDEEKEEYGLKPMNCPG
HCLIFGKKDRSYNELPLRFSDFSPLHRNEASGALSGLTRLRKFHQDDGHI
FCTPSQVKSEIFNSLKLIDIVYNKIFPSNYFINFSTRPDHFIGDLKVWNH
AEQVLKEILEESGKPWKLNPGDGAFYGPKLDIMVTDHLRKTHQVATIQLD
FQLPERFDLKFKDQDNSYKRPIMIHRATFGSIERFMALLIDSNEGRWPFW
LNPYQAVIIPVNTKNVQQLDMCTALQKKLRNELEADDMEPVPLNDWHFNV
DLDIRNEPVGYRIKSAILKNYSYLIIVGDEEVQLQKYNIRERDNKSFEKL
TMSQIWEKFIELEKNYK
Ligand information
>4yye Chain D (length=74) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guuauauuagcuuaauugguagagcauucguuuuguaaucgaaagguuug
ggguucaaaucccuaauauaacac
<<<<<<<..<<<<........>>>>.<<<<.........>>>>.....<<
<<<.......>>>>>>>>>>>>..
Receptor-Ligand Complex Structure
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PDB4yye The crystal structure of yeast mitochondrial ThrRS in complex with the canonical threonine tRNA.
Resolution2.301 Å
Binding residue
(original residue number in PDB)		Q44 P56 S166 G167 S170 G171 V355 N356 T357 N400 E401 P402 V403 G404 Y405 I407 K408 I411 I420 G422 E424 E425 N432 R434 F442
Binding residue
(residue number reindexed from 1)		Q9 P21 S131 G132 S135 G136 V311 N312 T313 N356 E357 P358 V359 G360 Y361 I363 K364 I367 I376 G378 E380 E381 N388 R390 F397
Enzymatic activity
Catalytic site (original residue number in PDB) C133 R162 Q180 D182 H184 K273 H319
Catalytic site (residue number reindexed from 1) C98 R127 Q145 D147 H149 K229 H275
Enzyme Commision number 6.1.1.3: threonine--tRNA ligase.
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004829 threonine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0140101 catalytic activity, acting on a tRNA
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006435 threonyl-tRNA aminoacylation
GO:0070159 mitochondrial threonyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4yye, PDBe:4yye, PDBj:4yye
PDBsum4yye
PubMed26704982
UniProtP07236|SYTM_YEAST Threonine--tRNA ligase, mitochondrial (Gene Name=MST1)

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