Structure of PDB 4yco Chain B Binding Site BS02

Receptor Information
>4yco Chain B (length=309) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRI
CPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGAP
SGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEI
ADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEI
WDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALL
QKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDI
ARAIQAIDI
Ligand information
>4yco Chain E (length=67) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gcggauagcucagucgguagagcaggggauugaaaauccccguguccuug
guucgauuccgaguccg
.<<<<..<<<<........>>>>.<<<<<<.....>>>>>>.....<<<<
<.......>>>>>>>>>
Receptor-Ligand Complex Structure
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PDB4yco Major reorientation of tRNA substrates defines specificity of dihydrouridine synthases.
Resolution2.1 Å
Binding residue
(original residue number in PDB)		E9 R35 Q39 P42 R48 A98 S106 Y176 A271 R272 K274 Y279 R295
Binding residue
(residue number reindexed from 1)		E10 R36 Q40 P43 R49 A99 S103 Y173 A268 R269 K271 Y276 R292
Enzymatic activity
Enzyme Commision number 1.3.1.-
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0050660 flavin adenine dinucleotide binding
GO:0102262 tRNA-dihydrouridine16 synthase activity
Biological Process
GO:0002943 tRNA dihydrouridine synthesis
GO:0008033 tRNA processing

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Molecular Function
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Biological Process
External links
PDB RCSB:4yco, PDBe:4yco, PDBj:4yco
PDBsum4yco
PubMed25902496
UniProtP33371|DUSC_ECOLI tRNA-dihydrouridine(16) synthase (Gene Name=dusC)

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