Structure of PDB 4r8u Chain B Binding Site BS02

Receptor Information

>4r8u Chain B (length=338) Species: 83333 (Escherichia coli K-12)

RKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKF
GVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLS
LDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKI
ASDMNKPNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCG
DVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVGVERTMAE
DIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQE
HVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLD

Ligand information

>4r8u Chain D (length=16)

tagggtcctaggaccc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4r8u Fast native-SAD phasing for routine macromolecular structure determination
• Resolution: 2.3 Å
• Binding residue (original residue number in PDB): E105 K151 G184 G186 V188 S189 R286 T299 T300 Q301 E302 H303 V304
• Binding residue (residue number reindexed from 1): E103 K149 G182 G184 V186 S187 R284 T297 T298 Q299 E300 H301 V302

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0005515 protein binding
• GO:0008296 3'-5'-DNA exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0000731 DNA synthesis involved in DNA repair
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0009432 SOS response
• GO:0042276 error-prone translesion synthesis
• GO:0070987 error-free translesion synthesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4r8u, PDBe:4r8u, PDBj:4r8u
• PDBsum: 4r8u
• PubMed: 25506719
• UniProt: Q47155|DPO4_ECOLI DNA polymerase IV (Gene Name=dinB)