Structure of PDB 4dsj Chain B Binding Site BS02

Receptor Information

>4dsj Chain B (length=575) Species: 1422 (Geobacillus stearothermophilus)

KMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFLR
PETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLLA
AYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHL
VRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDT
KRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPVL
KGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVRPDTKK
VHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESDWLIFA
ADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDEVTPNM
RRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGVKRYME
NIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPIQGSAA
DIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCRLVPEV
MEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>4dsj Chain F (length=10)

cctgactcgg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4dsj Biochemical and structural insights into the fidelity of bacillus stearothermophilus DNA polymerase
• Resolution: 2.86 Å
• Binding residue (original residue number in PDB): N527 N529 S530 S585 T586 L610 T611 Q612 T613 S617 T619 E620 N622 Y714 R771 R789 M790 Q797
• Binding residue (residue number reindexed from 1): N230 N232 S233 S284 T285 L309 T310 Q311 T312 S316 T318 E319 N321 Y413 R470 R488 M489 Q496

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0004518 nuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006302 double-strand break repair
• GO:0006974 DNA damage response
• GO:0071897 DNA biosynthetic process

External links

• PDB: RCSB:4dsj, PDBe:4dsj, PDBj:4dsj
• PDBsum: 4dsj
• UniProt: E1C9K5