Structure of PDB 3plf Chain B Binding Site BS02

Receptor Information

>3plf Chain B (length=301) Species: 9606 (Homo sapiens)

GTVDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTI
LSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPR
RNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKT
IVPWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLF
QPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCT
RLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPD
L

Ligand information

• Ligand ID: CA
• InChI: InChI=1S/Ca/q+2
• InChIKey: BHPQYMZQTOCNFJ-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Ca++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Ca+2]
• Formula: Ca
• Name: CALCIUM ION
• DrugBank: DB14577
• PDB chain: 3plf Chain B Residue 1

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3plf An adjacent arginine, and the phosphorylated tyrosine in the c-Met receptor target sequence, dictates the orientation of c-Cbl binding
• Resolution: 1.92 Å
• Binding residue (original residue number in PDB): D229 T231 N233 Y235 E240
• Binding residue (residue number reindexed from 1): D181 T183 N185 Y187 E192
• Annotation score: 4

Enzymatic activity

• Enzyme Commision number: 2.3.2.27: RING-type E3 ubiquitin transferase.

Gene Ontology

Molecular Function

• GO:0001784 phosphotyrosine residue binding
• GO:0004842 ubiquitin-protein transferase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0007166 cell surface receptor signaling pathway
• GO:0023051 regulation of signaling

External links

• PDB: RCSB:3plf, PDBe:3plf, PDBj:3plf
• PDBsum: 3plf
• PubMed: 21163258
• UniProt: P22681|CBL_HUMAN E3 ubiquitin-protein ligase CBL (Gene Name=CBL)