Structure of PDB 1j2b Chain B Binding Site BS02

Receptor Information
>1j2b Chain B (length=576) Species: 53953 (Pyrococcus horikoshii) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MLKFEIKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQMVVEPKELEKMG
FEIIITNSYIIYKDEELRRKALELGIHRMLDYNGIIEVDSGSFQLMKYGS
IEVSNREIIEFQHRIGVDIGTFLDIPTPPDAPREQAVKELEITLSRAREA
EEIKEIPMNATIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLESYRF
RDVVDIVISSKMALRPDRPVHLFGAGHPIVFALAVAMGVDLFDSASYALY
AKDDRYMTPEGTKRLDELDYFPCSCPVCSKYTPQELREMPKEERTRLLAL
HNLWVIKEEIKRVKQAIKEGELWRLVDERARSHPKLYSAYKRLLEHYTFL
EEFEPITKKSALFKISNESLRWPVVRRAKERAKSINERFGELVEHPIFGR
VSRYLSLTYPFAQSEAEDDFKIEKPTKEDAIKYVMAIAEYQFGEGASRAF
DDAKVELSKTGMPRQVKVNGKRLATVRADDGLLTLGIEGAKRLHRVLPYP
RMRVVVNKEAEPFARKGKDVFAKFVIFADPGIRPYDEVLVVNENDELLAT
GQALLSGREMIVFQYGRAVKVRKGVE
Ligand information
>1j2b Chain D (length=71) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gggcccguggucuaguugacgccgcccuuacgaggcggagguccgggguu
caaguccccgcgggcccacca
<<<<<<...........<<<.<<<<<.......>>>>>..>>><<<<<..
.....>>>>>.>>>>>>....
Receptor-Ligand Complex Structure
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PDB1j2b Alternative Tertiary Structure of tRNA for Recognition by a Posttranscriptional Modification Enzyme
Resolution3.3 Å
Binding residue
(original residue number in PDB)		A367 A418 D424 D425 K427 I428 S464 K465 T466 M468 Q471 R478 T481 A484 F519 F527 F530
Binding residue
(residue number reindexed from 1)		A361 A412 D418 D419 K421 I422 S458 K459 T460 M462 Q465 R472 T475 A478 F513 F521 F524
Enzymatic activity
Catalytic site (original residue number in PDB) D249 C279 C281 C284 H307
Catalytic site (residue number reindexed from 1) D243 C273 C275 C278 H301
Enzyme Commision number 2.4.2.48: tRNA-guanine(15) transglycosylase.
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0008270 zinc ion binding
GO:0016757 glycosyltransferase activity
GO:0016763 pentosyltransferase activity
GO:0046872 metal ion binding
Biological Process
GO:0002099 tRNA wobble guanine modification
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0008616 queuosine biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1j2b, PDBe:1j2b, PDBj:1j2b
PDBsum1j2b
PubMed12732145
UniProtO58843|ATGT_PYRHO tRNA-guanine(15) transglycosylase (Gene Name=tgtA)

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