Structure of PDB 8oin Chain Aa Binding Site BS02

Receptor Information

>8oin Chain Aa (length=381) Species: 9606 (Homo sapiens)

DTKMLWKHKALQKYMENLSKEYQTLEQCLQHIPVNEENRRSLNRRHAELA
PLAAIYQEIQETEQAIEELESMCKSLNKQDEKQLQELALEERQTIDQKIN
MLYNELFQSLVPKEKYDKNDVILEVTAGRTTGGDICQQFTREIFDMYQNY
SCYKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIP
EVGLSSRMQRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGAGGQH
VNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKD
KRQQQSARKLQVGTRAQSERIRTYNFTQDRVSDHRIAYEVRDIKEFLCGG
KGLDQLIQRLLQSADEEAIAELLDEHLKSAK

Ligand information

>8oin Chain AA (length=960)

acagguuugguccuggccuuucuauuaauucuuaauaaaauuacacaugc
aaguauccgcgccccggugagaaugcccuccagaucuuaaagaucaaaag
gagcagguaucaagcacaccuauaacgguagcucauaacgccuugcucaa
ccacacccccacgggaaacagcagugauaaaaauuaagccaugaacgaaa
guuugacuaaguuauauuaauuagaguugguaaaucucgugccagccacc
gcggucauacgauuaacccaaauuaauagauccacggcguaaagaguguu
uaagaaaaaaaaucacaauagaguuaaauuauaacuaagcuguaaaaagc
ccuaguuaaaauaaaauaacccacgaaagugacucuaauaauccugacac
acgauagcuaggacccaaacugggauuagauaccccacuaugccuagccc
uaaacccaaauaguuacauaacaaaacuauucgccagaguacuacucgca
acugccuaaaacucaaaggacuuggcggugcuucacauccaccuagagga
gccuguucuauaaucgauaaaccccgauagaccuuaccaacccuugccaa
uucagccuauauaccgccaucuucagcaaacccuaaaaaggaacaauagu
aagcacaaucauagcacauaaaaacguuaggucaagguguagcuuauggg
uuggaaagaaaugggcuacauuuucuacauaagaauauccaccacacgaa
aguuuuuaugaaacuaaaaaccaaaggaggauuuagcaguaaaucaagaa
uagagugcuugauugaauaaggccaugaagcacgcacacaccgcccguca
cccuccucaagcauguaguaauaaaaauaaccuauauucaauuacacaac
caugcaagaagagacaagucguaacaagguaagcauacuggaaagugugc
uuggauuacc

..((((..<<<<<<.)))).<<<<<<<<<..<<<<<<<...<.<<<.<<<
..<<....>>......<<......<<<<<...<<<<<...>>>>>...>>
>.>>.<<<....<<...<<<......>>>..>>......>>>..<.....
>.>>.<<<....>>>.....>>>>>>>.........<<....<<<<....
>>>>..>>....>.>>>>>>...<.<<<<<<.....(((.....((.>>>
)).......)))..>>>.>..>>>>>>>>>.....<<<((.....<<<<.
..<<..............<<<<<<.....<.<<<<<<.<<<......>>>
..>>>>>>..>..........<<<....>>>>>>>>>......>>...>>
>>....<<<<<<...<<...<<<<.<.....>.>>>>...>>>>>>>>..
........<<<<<<<.....))..>>>>>>>.>>>.<<<........<<.
...>>......>>>..>>>>>><<<<<.<<<<<<<...<<..<<<<<..<
<<<<<<<<<.....<<........>>..........<<<<<<<.......
...<<<.<<<..<<<<<<.......<<..<<<<<....>>>.......>>
..>>......<<.....>>..........>>>...>>>>>>>>>...>>>
>>>>..>>.>>>>>>>>.....<<<<.....>>>>...............
.<<<<<<<......>>>>>>>..........>>>>>.....<<<<<<<..
.......>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.
..<<<.<<..<<<<<..<<<<<...<<<<.....>>>>..>>>>>.....
>>>>>..>>.>>>....>.>>...>.....<<<<<<<<<....>>>>>>>
>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8oin Molecular basis of translation termination at noncanonical stop codons in human mitochondria.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): R193 T194 T195 G196 H236 R259 Q261 R271 Q273 R274 I275 T378 R379 R384 T391 Q392
• Binding residue (residue number reindexed from 1): R129 T130 T131 G132 H172 R195 Q197 R207 Q209 R210 I211 T314 R315 R320 T327 Q328

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0016149 translation release factor activity, codon specific

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination
• GO:0070126 mitochondrial translational termination

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion

External links

• PDB: RCSB:8oin, PDBe:8oin, PDBj:8oin
• PDBsum: 8oin
• PubMed: 37141370
• UniProt: O75570|RF1M_HUMAN Peptide chain release factor 1, mitochondrial (Gene Name=MTRF1)