Structure of PDB 8wx0 Chain A Binding Site BS02

Receptor Information

>8wx0 Chain A (length=597) Species: 1773 (Mycobacterium tuberculosis)

MSAAEIDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDN
MLLSATTASKNPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDA
ILTCRLIDRPLRPSFVDGLRNEIQIVVTILSLDPGDLYDVLAINAASAST
QLGGLPFSGPIGGVRVALIDGTWVGFPTVDQIERAVFDMVVAGRIVEGDV
AIMMVEAEATENVVELVEGGAQAPTESVVAAGLEAAKPFIAALCTAQQEL
ADAAGKSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQ
RIDEIKTQVVQRLADTYEGREKEVGAALRALTKKLVRQRILTDHFRIDGR
GITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLG
PETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVE
EFPYAIRQVSEALGSNGSTSMGSVCASTLALLNAGVPLKAPVAGIAMGLV
SDDIQVEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQL
DTKLDGIPSQVLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRV

Ligand information

>8wx0 Chain D (length=3)

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 8wx0 Cryo-EM structures of Mycobacterium tuberculosis polynucleotide phosphorylase suggest a potential mechanism for its RNA substrate degradation.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): F66 F68 L71 T72 R423 R429 R430
• Binding residue (residue number reindexed from 1): F66 F68 L71 T72 R423 R429 R430

Gene Ontology

Molecular Function

• GO:0000175 3'-5'-RNA exonuclease activity
• GO:0000287 magnesium ion binding
• GO:0003723 RNA binding
• GO:0004654 polyribonucleotide nucleotidyltransferase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006401 RNA catabolic process
• GO:0006402 mRNA catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:8wx0, PDBe:8wx0, PDBj:8wx0
• PDBsum: 8wx0
• PubMed: 38395123
• UniProt: P9WI57|PNP_MYCTU Polyribonucleotide nucleotidyltransferase (Gene Name=pnp)