Structure of PDB 8uza Chain A Binding Site BS02

Receptor Information
>8uza Chain A (length=910) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MRYKIGLAIGITSVGWAVMNLDIPRIEDLGVRIFDRAENPQTGESLALPR
RLARSARRRLRRRKHRLERIRRLVIREGILTKEELDKLFEEKHEIDVWQL
RVEALDRKLNNDELARVLLHLAKRRGFKSNRKSSTMLKHIEENRAILSSY
RTVGEMIVKDPKFALHKRNKGENYTNTIARDDLEREIRLIFSKQREFGNM
SCTEEFENEYITIWASQRPVASKDDIEKKVGFCTFEPKEKRAPKATYTFQ
SFIAWEHINKLRLISPSGARGLTDEERRLLYEQAFQKNKITYHDIRTLLH
LPDDTYFKGIVYDRGESRKQNENIRFLELDAYHQIRKAVDKVYGKGKSSS
FLPIDFDTFGYALTLFKDDADIHSYLRNEYEQNGKRMPNLANKVYDNELI
EELLNLSFTKFGHLSLKALRSILPYMEQGEVYSSACERAGYTFTGPKKKQ
KTMLLPNIPPIANPVVMRALTQARKVVNAIIKKYGSPVSIHIELARDLSQ
TFDERRKTKKEQDENRTRYISRFFANFIREHLKFAESDDKQKVYTVNGRV
TAHLRSRWEFNKNREESDLHHAVDAVIVACTTPSDIAKVTAFYQRREQNE
PHFPQPWPHFADELRARLSKHPKESIKALNLGNYDDQKLESLQPVFVSRM
PKRSVTGAAHQETLRRYVGIDERSGKIQTVVKTKLSEIKLDASGHFPMYG
KESDPRTYEAIRQRLLEHNNDPKKAFQEPLYKPKKNGEPGPVIRTVKIID
TKNQVIPLNDGKTVAYNSNIVRVDVFEKDGKYYCVPVYTMDIMKGILPNK
AIEPNKPYSEWKEMTEDYTFRFSLYPNDLIRIELPREKTVKTAAGEEINV
KDVFVYYKTIDSANGGLELISHDHRFSLRGVGSRTLKRFEKYQVDVLGNI
YKVRGEKRVG
Ligand information
>8uza Chain C (length=44) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ttgatgagtttggacaaaccacaactagaatgcagtgaaaaaaa
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB8uza Accelerated DNA unwinding supercharges genome editing by engineered CRISPR-Cas9
Resolution3.17 Å
Binding residue
(original residue number in PDB)		F127 S129 Y181 K236 V237 R248 K267 K315 K374 T416 F418 S440 F450 E818 T819 N1020 R1035 G1036 V1037 G1038 R1040 T1041
Binding residue
(residue number reindexed from 1)		F127 S129 Y174 K229 V230 R241 K260 K308 K367 T409 F411 S433 F443 E662 T663 N864 R879 G880 V881 G882 R884 T885
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8uza, PDBe:8uza, PDBj:8uza
PDBsum8uza
PubMed38781968
UniProtA0A150MP45

[Back to BioLiP]