Structure of PDB 8uc2 Chain A Binding Site BS02

Receptor Information

>8uc2 Chain A (length=339) Species: 319 (Pseudomonas savastanoi pv. phaseolicola)

NLQTFELPTEVTGCAADISLGRALIQAWQKDGIFQIKTDSEQDRKTQEAM
AASKQFCKEPLTFKSSCVSDLTYSGYVASGEEKPDFPEIFTVCKDLSVGD
QRVKAGWPCHGPVPWPNNTYQKSMKTFMEELGLAGERLLKLTALGFELPI
NTFTDLTRDGWHHMAVLRFPPQTSTLSRGIGAHTDYGLLVIAAQDDVGGL
YIRPPVEGEKRNRNWLPGESSAGMFEHDEPWTFVTPTPGVWTVFPGDILQ
FMTGGQLLSTPHKVKLNTRERFACAYFHEPNFEASAYPLFEPSANERIHY
GEHFTNMFMRCYPDRITTQRINKENRLAHLEDLKKYSDT

Ligand information

• Ligand ID: CA
• InChI: InChI=1S/Ca/q+2
• InChIKey: BHPQYMZQTOCNFJ-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Ca++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Ca+2]
• Formula: Ca
• Name: CALCIUM ION
• DrugBank: DB14577
• PDB chain: 8uc2 Chain A Residue 405

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8uc2 Ethylene forming enzyme (EFE) R171A variant in complex with nickel and Benzoic acid
• Resolution: 1.6 Å
• Binding residue (original residue number in PDB): N220 L222 E225
• Binding residue (residue number reindexed from 1): N214 L216 E219
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 1.13.12.19: 2-oxoglutarate dioxygenase (ethylene-forming).
  1.14.20.7: 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate- forming).

Gene Ontology

Molecular Function

• GO:0016706 2-oxoglutarate-dependent dioxygenase activity
• GO:0046872 metal ion binding
• GO:0051213 dioxygenase activity
• GO:0102276 2-oxoglutarate oxygenase/decarboxylase (ethylene-forming) activity

Biological Process

• GO:0009693 ethylene biosynthetic process

External links

• PDB: RCSB:8uc2, PDBe:8uc2, PDBj:8uc2
• PDBsum: 8uc2
• PubMed: 38577885
• UniProt: P32021|EFE_PSESH 2-oxoglutarate-dependent ethylene/succinate-forming enzyme (Gene Name=efe)