Structure of PDB 8sfl Chain A Binding Site BS02

Receptor Information
>8sfl Chain A (length=1296) Species: 1111120 (Acidaminococcus sp. BV3L6) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKEL
KPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQA
TYRNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVT
TTEHENALLRSFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPK
FKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEVFSFPFYNQLL
TQTQIDLYNQLLGGIEKIKGLNEVLNLAIQKNDETAHIIASLPHRFIPLF
KQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALFNEL
NSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELKSAKEKVQR
SLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQ
EEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSL
SFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNG
LYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQ
LKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAK
KTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYKDLGEYY
AELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHHGKPNLH
TLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKL
KDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKD
RRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGE
RNLIYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVV
GTIKDLKQGYLSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAV
YQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGF
LFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDVKTG
DFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIV
PVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHA
IDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMD
ADANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN
Ligand information
>8sfl Chain C (length=40) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
actccagtacgcattccacttatcactaaaagatcggaag
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB8sfl WT CRISPR-Cas12a with a 15bp R-loop
Resolution3.3 Å
Binding residue
(original residue number in PDB)		E174 N178 I185 S186 F318 L320 K403 S404 N515 R518 N519 G543 D545 N547 K548 Y597 P599 M604 K607 L612 K613 K780 N782 G783 Q784 S1051 F1052 N1090
Binding residue
(residue number reindexed from 1)		E174 N178 I185 S186 F312 L314 K392 S393 N504 R507 N508 G532 D534 N536 K537 Y586 P588 M593 K596 L601 K602 K769 N771 G772 Q773 S1040 F1041 N1079
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004530 deoxyribonuclease I activity
GO:0016829 lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8sfl, PDBe:8sfl, PDBj:8sfl
PDBsum8sfl
PubMed
UniProtU2UMQ6|CS12A_ACISB CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

[Back to BioLiP]