Structure of PDB 8pj9 Chain A Binding Site BS02

Receptor Information
>8pj9 Chain A (length=1051) Species: 1268061 (Streptococcus thermophilus DGCC 7710) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KPYSIGLDIGTNSVGWAVITDNYKVPSKKMKVLGNTSKKYIKKNLLGVLL
FDSGITAEGRRLKRTARRRYTRRRNRILYLQEIFSTEMATLDDAFFQRLD
DSFLVPDDKRDSKYPIFGNLVEEKVYHDEFPTIYHLRKYLADSTKKADLR
LVYLALAHMIKYRGHFLIEGEFNVTDNETEAPLSSAMIKRYNEHKEDLAL
LKEYIRNISLKTYNEVFKDDTKNGYAGYIDGKTNQEDFYVYLKNLLAEFE
GADYFLEKIDREDFLRKQRTFDNGSIPYQIHLQEMRAILDKQAKFYPFLA
KNKERIEKILTFRIPYYVGPLARGNSDFAWSIRKRNEKITPWNFEDVIDK
ESSAEAFINRMTSFDLYLPEEKVLPKHSLLYETFNVYNELTKVRFIAESM
RDYQFLDSKQKKDIVRLYFKDKRKVTDKDIIEYLHAIYGYDGIELKGIEK
QFNSSLSTYHDLLNIINDKEFLDDSSNEAIIEEIIHTLTIFEDREMIKQR
LSKFENIFDKSVLKKLSRRHYTGWGKLSAKLINGIRDEKSGNTILDYLID
DGISNRNFMQLIHDDALSFKKKIQKAQIIGDEDKGNIKEVVKSLPGSPAI
KKGILQSIKIVDELVKVMGGRKPESIVVEMATKHVARLLDEKFNNKKDEN
NRAVRTVKIITLKSTLVSQFRKDFELYKVREINDFHHAHDAYLNAVIASA
LLKKYPKLEPEFVYGSNIMNIFKKSRPLIEVNEETGESVWNKESDLATVR
RVLSYPQVNVVKKVEEQNHGLDRGKPKGLFNANLSSKPKPNSNENLVGAK
EYLDPKKYGGYAGISNSFAVLVKGTIEKGAKKKITNVLEFQGISILDRIN
YRKDKLNFLLEKGYKDIELIIELPKYSLFELSDGSRRMLASILSTNNKRG
EIHKGNQIFLSQKFVKLLYHAKRISNTINENHRKYVENHKKEFEELFYYI
LEFNENYVGAKKNGKLLNSAFQSWQNHSIDELCSSFIGPTGSERKGLFEL
TSRGSAADFEFLGVKIPRYRDYTPSSLLKDATLIHQSVTGLYETRIDLAK
L
Ligand information
>8pj9 Chain C (length=66) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aacacagcgaguuaaaauaaggcuuaguccguacucaacuugaaaaggug
gcaccgauucgguguu
....................<<......>>.......<<<......>>>.
<<<<<<...>>>>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8pj9 Structural basis for spacer acquisition in a type II-A CRISPR-Cas system
Resolution3.24 Å
Binding residue
(original residue number in PDB)
S29 K30 K33 L35 Y42 K44 N46 I57 R62 K65 R66 R69 R70 Y72 R74 R75 R76 N77 L101 S104 F105 T134 I135 H160 K163 Y325 H328 K336 R340 L344 F351 K352 Y359 I363 Q402 R403 F446 L455 A456 R457 N459 S460 K472 I473 N720 K736 L739 Q740 K743 Y1089 P1090 Q1091 N1102 L1113 F1114 A1133 K1134 E1135 L1180 R1220 Q1241 I1242 F1243 H1369 Q1370 S1371 Y1376
Binding residue
(residue number reindexed from 1)
S27 K28 K31 L33 Y40 K42 N44 I55 R60 K63 R64 R67 R68 Y70 R72 R73 R74 N75 L99 S102 F103 T132 I133 H158 K161 Y191 H194 K202 R206 L210 F217 K218 Y225 I229 Q268 R269 F312 L321 A322 R323 N325 S326 K338 I339 N586 K602 L605 Q606 K609 Y755 P756 Q757 N768 L779 F780 A799 K800 E801 L846 R886 Q907 I908 F909 H1035 Q1036 S1037 Y1042
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function

View graph for
Biological Process
External links
PDB RCSB:8pj9, PDBe:8pj9, PDBj:8pj9
PDBsum8pj9
PubMed
UniProtG3ECR1|CAS9_STRTR CRISPR-associated endonuclease Cas9 (Gene Name=cas9)

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